We investigated serological responses following a SARS-CoV-2 outbreak in spring 2020 on a US Marine recruit training base. 147 participants that were isolated during an outbreak of respiratory illness were enrolled in this study, with visits approximately 6 and 10 weeks post-outbreak (PO). This cohort is comprised of young healthy adults, ages 18-26, with a high rate of asymptomatic infection or mild symptoms, and therefore differs from previously reported longitudinal studies on humoral responses to SARS-CoV-2, which often focus on more diverse age populations and worse clinical presentation. 80.9% (119/147) of the participants presented with circulating IgG antibodies against SARS-CoV-2 spike (S) receptor-binding domain (RBD) at 6 weeks PO, of whom 97.3% (111/114) remained positive, with significantly decreased levels, at 10 weeks PO. Neutralizing activity was detected in all sera from SARS-CoV-2 IgG positive participants tested (n=38) at 6 and 10 weeks PO, without significant loss between time points. IgG and IgA antibodies against SARS-CoV-2 RBD, S1, S2, and the nucleocapsid (N) protein, as well neutralization activity, were generally comparable between those participants that had asymptomatic infection or mild disease. A multiplex assay including S proteins from SARS-CoV-2 and related zoonotic and human endemic betacoronaviruses revealed a positive correlation for polyclonal cross-reactivity to S after SARS-CoV-2 infection. Overall, young adults that experienced asymptomatic or mild SARS-CoV-2 infection developed comparable humoral responses, with no decrease in neutralizing activity at least up to 10 weeks after infection.
【저자키워드】 COVID-19, antibodies, SARS-CoV-2, outbreak, young adults, 【초록키워드】 IgG, S protein, SARS-COV-2 infection, Infection, Respiratory illness, Betacoronavirus, Population, cross-reactivity, Protein, Adults, Neutralizing activity, Cohort, IgG antibody, Multiplex assay, Humoral response, Asymptomatic, IgG antibodies, longitudinal study, nucleocapsid, RBD, sera, zoonotic, Longitudinal studies, asymptomatic infection, Mild, age, humoral responses, respiratory, disease, SARS-CoV-2 IgG, mild symptoms, Mild disease, SARS-CoV-2 spike, serological response, Endemic, SARS-CoV-2 RBD, focus, spring, positive correlation, IgA antibodies, domain, S proteins, participant, neutralization activity, circulating, positive, mild SARS-CoV-2 infection, no decrease, polyclonal, enrolled, tested, significantly, healthy, reported, investigated, remained, comparable, IgA antibody, recruit, 【제목키워드】 response,