Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) pandemic is still ongoing along with the global vaccination efforts against it. Here, we aimed to understand the longevity and strength of anti-SARS-CoV-2 IgG responses in a small community ( n = 283) six months following local SARS-COV-2 outbreak in March 2020. Three serological assays were compared and neutralisation capability was also determined. Overall 16.6% (47/283) of the participants were seropositive and 89.4% (42/47) of the IgG positives had neutralising antibodies. Most of the symptomatic individuals confirmed as polymerase chain reaction (PCR) positive during the outbreak were seropositive (30/32, 93.8%) and 33.3% of the individuals who quarantined with a PCR confirmed patient had antibodies. Serological assays comparison revealed that Architect (Abbott) targeting the N protein LIASON ® (DiaSorin) targeting the S protein and enzyme-linked immunosorbent assay (ELISA) targeting receptor binding domain detected 9.5% (27/283), 17.3% (49/283) and 17% (48/283), respectively, as IgG positives. The latter two assays highly agreed (kappa = 0.89) between each other. In addition, 95%, (19/20, by ELISA) and 90.9% (20/22, with LIASON) and only 71.4% (15/21, by Architect) of individuals that were seropositive in May 2020 were found positive also in September. The unexpected low rate of overall immunity indicates the absence of un-noticed, asymptomatic infections. Lack of overall high correlation between the assays is attributed mainly to target-mediated antibody responses and suggests that using a single serological assay may be misleading.
【저자키워드】 SARS-CoV-2, Neutralising Antibodies, Sero-prevalence, IgG antibodies, herd immunity, 【초록키워드】 antibodies, IgG, vaccination, pandemic, Immunity, S protein, Antibody Response, Neutralising Antibodies, Local, ELISA, Receptor binding domain, asymptomatic infections, Severe acute respiratory syndrome, polymerase chain reaction, enzyme-linked immunosorbent assay, PCR, Serological assay, outbreak, anti-SARS-CoV-2 IgG, serological assays, response, Patient, N protein, Community, respiratory, Longevity, seropositive, severe acute respiratory syndrome-coronavirus-2, neutralisation, Abbott, IgG responses, Chain Reaction, (Abbott, strength, enzyme, individual, DiaSorin, high correlation, symptomatic individuals, effort, participant, Architect, positive, MOST, enzyme-linked immunosorbent, polymerase chain, addition, indicate, absence, the S protein, the N protein, symptomatic individual, 【제목키워드】 outbreak, anti-SARS-CoV-2 IgG, response,