Abstract A deeper understanding of COVID‐19 on human molecular pathophysiology is urgently needed as a foundation for the discovery of new biomarkers and therapeutic targets. Here we applied mass spectrometry (MS)‐based proteomics to measure serum proteomes of COVID‐19 patients and symptomatic, but PCR‐negative controls, in a time‐resolved manner. In 262 controls and 458 longitudinal samples of 31 patients, hospitalized for COVID‐19, a remarkable 26% of proteins changed significantly. Bioinformatics analyses revealed co‐regulated groups and shared biological functions. Proteins of the innate immune system such as CRP, SAA1, CD14, LBP, and LGALS3BP decreased early in the time course. Regulators of coagulation (APOH, FN1, HRG, KNG1, PLG) and lipid homeostasis (APOA1, APOC1, APOC2, APOC3, PON1) increased over the course of the disease. A global correlation map provides a system‐wide functional association between proteins, biological processes, and clinical chemistry parameters. Importantly, five SARS‐CoV‐2 immunoassays against antibodies revealed excellent correlations with an extensive range of immunoglobulin regions, which were quantified by MS‐based proteomics. The high‐resolution profile of all immunoglobulin regions showed individual‐specific differences and commonalities of potential pathophysiological relevance. A total of 720 proteomes from 458 longitudinal serum samples of hospitalized COVID‐19 cases and 262 symptomatic controls were measured. In‐depth analysis revealed regulation of innate immune and coagulation systems and individual‐specific trajectories of immunoglobulin regions.
【저자키워드】 proteomics, Biomarker, Biomarkers, immunoglobulins, SARS‐CoV‐2, Microbiology, Virology & Host Pathogen Interaction, biobanking, individual‐specific, 【초록키워드】 Biological functions, mass spectrometry, Hospitalized, antibody, CRP, Proteins, COVID‐19, SARS‐CoV‐2, Coagulation, innate immune system, Protein, immunoassay, serum, Region, Immunoglobulin, pathophysiology, symptomatic, Control, trajectory, Coagulation system, proteome, molecular, correlation, group, patients, therapeutic targets, clinical chemistry, association, innate immune, Analysis, KNG1, Regulation, biological processes, COVID‐19 patients, foundation, CD14, regulator, apoA1, serum sample, Saa1, LGALS3BP, longitudinal samples, PON1, regions, FIVE, controls, APOC1, APOC2, APOC3, APOH, clinical chemistry parameters, lipid homeostasis, commonality, Course, significantly, the disease, applied, functional, provide, changed, were measured, quantified, COVID‐19 patient, FN1, HRG, LBP, pathophysiological, PLG, 【제목키워드】 COVID‐19, serum, Seroconversion, trajectory, proteome,