Abstract
The level of neutralizing antibody (NAb) to SARS-CoV-2 could be used to evaluate the acquired protective immunity of COVID-19 patients or vaccinees. Here we develop a track-etched microporous membrane filtration microplate (TEM) and optical fibers transmitted immunosensing smartphone platform (TEMFIS) based surrogate virus neutralization test (TEMFIS-sVNT) for rapid one-step testing of NAb to SARS-CoV-2. Coefficient variation (CV) of intra-assay and inter-assay precisions of TEMFIS-sVNT varied below 9% or 14%, respectively. By agreement with pseudovirus neutralization test (pVNT) and ELISA-sVNT for testing of serum samples from 41 COVID-19 patients, 50 COVID-19 vaccinees and 320 healthy blood donors (P = 0.895), TEMFIS-sVNT detected the NAb positivity (sensitivity) in 92.68% COVID-19 patients and 76% vaccinees, but the NAb negativity (specificity) in 100% blood donors. In conclusion, TEMFIS-sVNT can be used for quantitatively point-of-care testing of neutralizing antibody to SARS-CoV-2 in blood samples from COVID-19 patients and vaccinees.
Keywords: COVID-19 patients; Neutralizing antibody; Optical fiber; Smartphone; Track-etched membrane; Vaccinees.
【저자키워드】 neutralizing antibody, Smartphone, COVID-19 patients, Optical fiber, Track-etched membrane, Vaccinees., 【초록키워드】 COVID-19, neutralizing antibody, SARS-CoV-2, neutralization, Variation, ELISA, point-of-care, sensitivity, specificity, Blood donors, protective immunity, pseudovirus, virus neutralization test, neutralization test, Smartphone, membrane, optical, platform, COVID-19 patients, Optical fiber, surrogate virus neutralization test, COVID-19 patient, Optical Fibers, Precision, serum samples, Blood samples, sVNT, blood sample, coefficient, negativity, vaccinees, serum sample, filtration, TEM, fiber, develop, transmitted, can be used, COVID-19 vaccinee, healthy blood donor, used to evaluate, 【제목키워드】 membrane, SARS-CoV-2 neutralizing antibody,