Abstract
In SLE, underlying immune dysregulation and immunosuppression may increase the susceptibility to COVID-19 and impair the humoral and adaptive response. We aimed to characterize COVID-19 infection, identifying susceptibility and severity risk factors, assessing the presence of SARS-CoV-2 IgG antibodies and analyzing the cellular response. We established a prospective cohort of lupus patients to estimate the COVID-19 incidence compared to the reference general population. Data were collected via telephone interviews and medical record review. SARS-CoV-2 IgG antibodies were measured cross-sectionally as part of routine surveillance. Longitudinal changes in antibody titers and immunological profile from convalescent COVID-19 patients were evaluated at 6, 12 and 24 week after symptom onset. From immunological studies, PBMCs from convalescent patients were extracted and analyzed by flow cytometry and gene expression analysis. We included 725 patients, identifying 29 with PCR-confirmed COVID-19 infection and 16 with COVID-19-like symptoms without PCR-testing. Of the 29 confirmed cases, 7 had severe disease, 8 required hospital admission (27.6%), 4 intensive care, and 1 died. COVID-19 accumulated incidence was higher in lupus patients. Health care workers and anti-SSA/Ro52 antibody positivity were risk factors for COVID-19 susceptibility, and hypocomplementemia for severity. SARS-CoV-2 IgG antibodies were detected in 8.33% of patients. Three fourths of confirmed COVID-19 cases developed antibodies. High prednisone doses were associated with lack of antibody response. Antibody titers declined over time (39%). Convalescent patients at week 12 after symptom onset displayed a CD8 + T cell reduction and predominant Th17 with a mild Th2 response, more pronounced in severe COVID-19 disease. Longitudinal immune response analysis showed a progressive sustained increase in CD8 + T and B memory cells with a decrease of Th17 signaling. Lupus patients are at higher risk of COVID-19 infection and new susceptibility and severity risk factors were identified. Lupus patients were able to mount humoral and cellular responses despite immunosuppressive therapy.
【초록키워드】 COVID-19, antibodies, immune response, therapy, Risk factors, Health care, adaptive, intensive care, antibody, susceptibility, severity, Antibody Response, Th17, Infection, Immunosuppression, Symptom, risk factor, CD8, flow cytometry, COVID-19 disease, T cell, Convalescent patients, Surveillance, COVID-19 infection, IgG antibodies, SARS-CoV-2 IgG antibody, Gene expression analysis, Patient, Antibody titer, Mild, PBMC, Prednisone, Hospital admission, General population, incidence, Lupus, prospective cohort, Th2 response, longitudinal, patients, SARS-CoV-2 IgG, severe COVID-19 disease, Antibody titers, convalescent patient, immunosuppressive therapy, COVID-19 susceptibility, convalescent COVID-19 patients, cellular response, Signaling, Analysis, dose, PBMCs, severe disease, immunosuppressive, immune dysregulation, humoral, SLE, memory cell, Humoral and cellular responses, dysregulation, medical record, longitudinal changes, reduction, symptom onset, COVID-19 incidence, higher risk, immunological profile, confirmed cases, SARS-CoV-2 IgG antibodies, PCR-confirmed COVID-19, Immunological studies, confirmed COVID-19 case, immunological, decrease, hypocomplementemia, analyzed, lack, collected, died, evaluated, required, changes in, increase in, predominant, sustained, impair, were measured, accumulated, declined, convalescent COVID-19 patient, 【제목키워드】 COVID-19, Patient, convalescent,