Abstract
Elucidating the characteristics of human immune response against SARS-CoV-2 is of high priority and relevant for determining vaccine strategies. We report the results of a follow-up evaluation of anti-SARS-CoV-2 antibodies in 148 convalescent plasma donors who participated in a phase 2 study at a median of 8.3 months (range 6.8-10.5 months) post first symptom onset. Monitoring responses over time, we found contraction of antibody responses for all four antigens tested, with Spike antibodies showing higher persistence than Nucleocapsid antibodies. A piecewise linear random-effects multivariate regression analysis showed a bi-phasic antibody decay with a more pronounced decrease during the first 6 months post symptoms onset by analysis of two intervals. Interestingly, antibodies to Spike showed better longevity whereas their neutralization ability contracted faster. As a result, neutralizing antibodies were detected in only 76% of patients at the last time point. In a multivariate analysis, older age and hospitalization were independently associated with higher Spike, Spike-RBD, Nucleocapsid, N-RBD antibodies and neutralizing antibody levels. Results on persistence and neutralizing ability of anti-SARS-CoV-2 antibodies, especially against Spike and Spike-RBD, should be considered in the design of future vaccination strategies.
Keywords: Antibodies; COVID-19; Immunity; Neutralizing; SARS-CoV-2; Spike.
【저자키워드】 COVID-19, antibodies, SARS-CoV-2, Immunity, spike, Neutralizing, 【초록키워드】 convalescent plasma, neutralizing antibody, Vaccine, Hospitalization, Neutralizing antibodies, spike, antibody, Phase 2, Antibody Response, Antigen, anti-SARS-CoV-2 antibodies, Antibody responses, Characteristics, anti-SARS-CoV-2 antibody, nucleocapsid, persistence, response, Vaccination strategies, Patient, Older age, antigens, monitoring, Neutralizing, Follow-up, multivariate regression analysis, Donor, Phase 2 study, Multivariate analysis, Analysis, regression analysis, symptom onset, symptoms onset, contraction, human immune response, intervals, neutralization ability, decrease, Result, tested, linear, median, faster, participated, random-effect, 【제목키워드】 convalescent plasma, neutralizing antibody, antibody, SARS-CoV-2 antibody, Donor, eight,