Abstract
COVID-19 remains as a major threat to human society. A reliable, sensitive, rapid, and low requirement assay for serum neutralizing antibodies is needed as a pandemic management tool for estimation of revaccination time and implementation of “immune passport”. Using gold nanoparticle (AuNR) as an immunosensor, we have established a semi-quantitative, instrument-free assay for measuring antibody level against SRAS-CoV-2 spike1 (S1) receptor binding domain (RBD) from fingertip blood samples. The testing results by the developed method correlated well with those obtained from conventional ELISA assay, indicating reliable quantitation could be achieved without use of plate reader. A declined of immunoglobulin G (IgG) antibody associated with vaccination time was observed, which agreed well with the data from other reports. The developed method provides a potentially complementary strategy for on-site measurement of COVID-19 antibodies.
Keywords: AuNR; Colorimetric reagent; Fingertip blood; IgG antibody; SARS-CoV-2.
【저자키워드】 SARS-CoV-2, IgG antibody, AuNR, Colorimetric reagent, Fingertip blood, 【초록키워드】 COVID-19, antibodies, SARS-CoV-2, IgG, vaccination, pandemic, antibody, Receptor binding domain, Immunoglobulin G, IgG antibody, Immunoglobulin, RBD, management, implementation, gold, estimation, Colorimetric reagent, Fingertip blood, ELISA assay, Gold nanoparticle, SRAS-CoV-2, complementary, fingertip, revaccination, quantitation, Serum neutralizing antibody, plate reader, provide, correlated, semi-quantitative, declined, 【제목키워드】 IgG, COVID-19 antibody,