Abstract
SARS-CoV-2 infection or vaccination produces neutralizing antibody responses that contribute to better clinical outcomes. The receptor-binding domain (RBD) and the N-terminal domain (NTD) of the spike trimer (S) constitute the two major neutralizing targets for antibodies. Here, we use NTD-specific probes to capture anti-NTD memory B cells in a longitudinal cohort of infected individuals, some of whom were vaccinated. We found 6 complementation groups of neutralizing antibodies. 58% targeted epitopes outside the NTD supersite, 58% neutralized either Gamma or Omicron, and 14% were broad neutralizers that also neutralized Omicron. Structural characterization revealed that broadly active antibodies targeted three epitopes outside the NTD supersite including a class that recognized both the NTD and SD2 domain. Rapid recruitment of memory B cells producing these antibodies into the plasma cell compartment upon re-infection likely contributes to the relatively benign course of subsequent infections with SARS-CoV-2 variants, including Omicron.
Keywords: SARS-CoV-2; anti-NTD neutralizing antibodies.
【저자키워드】 SARS-CoV-2, anti-NTD neutralizing antibodies., 【초록키워드】 antibodies, vaccination, Neutralizing antibodies, antibody, SARS-COV-2 infection, Infection, memory B cells, omicron, clinical outcomes, Epitopes, Cohort, Receptor-binding domain, SARS-CoV-2 variants, RBD, Re-infection, Rapid, Gamma, plasma, target, Neutralizing, NTD, recruitment, group, epitope, Neutralizing antibody response, Plasma cell, memory B cell, N-terminal domain, capture, infected individuals, domain, neutralizing antibody responses, class, subsequent infections, spike trimer, probe, Subsequent infection, Cell, targeted epitope, neutralized, Course, contribute, producing, 【제목키워드】 variant, Spike protein, neutralizing epitope, memory B cell, identify, conserved,