Abstract
To evaluate vaccine-induced humoral and cell-mediated immunity at 6 months after completion of two doses of BNT162b2 vaccination, immunoglobulin G against SARS-CoV-2 spike protein (SP IgG), 50% neutralizing antibody (NT 50 ), and spot-forming cell (SFC) counts were evaluated by interferon-γ releasing ELISpot assay of 98 healthy subjects (median age, 43 years). The geometric mean titers of SP IgG and NT 50 decreased from 95.2 (95% confidence interval (CI) 79.8-113.4) to 5.7 (95% CI 4.9-6.7) and from 680.4 (588.0-787.2) to 130.4 (95% CI 104.2-163.1), respectively, at 3 weeks and 6 months after the vaccination. SP IgG titer was negatively correlated with age and alcohol consumption. Spot-forming cell counts at 6 months did not correlate with age, gender, and other parameters of the patients. SP IgG, NT 50, and SFC titers were elevated in the breakthrough infected subjects. Although the levels of vaccine-induced antibodies dramatically declined at 6 months after vaccination, a certain degree of cellular immunity was observed irrespective of the age.
Keywords: 50% neutralization titer; Cell-mediated immunity; Coronavirus disease-2019; Interferon-γ releasing ELISpot assay; Vaccination; Variant of concern.
【저자키워드】 vaccination, coronavirus disease-2019, variant of concern, 50% neutralization titer, Cell-mediated immunity, Interferon-γ releasing ELISpot assay, 【초록키워드】 coronavirus disease, neutralizing antibody, IgG, coronavirus, neutralization, alcohol consumption, variant, interferon, Gender, alcohol, Spike protein, coronavirus disease-2019, BNT162b2, Immunoglobulin G, Immunoglobulin, cellular immunity, SARS-CoV-2 spike protein, age, ELISPOT, patients, ELISpot assay, Cell-mediated immunity, dose, humoral, geometric mean, interferon-γ, 95% CI, 95% confidence interval, vaccine-induced antibodies, median age, geometric mean titers, completion, geometric mean titer, neutralization titer, cell count, parameter, SFC, Cell, evaluate, evaluated, elevated, subjects, correlated, healthy subject, declined, of BNT162b2, vaccine-induced antibody, 【제목키워드】 SARS-CoV-2, humoral, remained, declined,