Abstract
COVID-19 vaccination efficacy depends on serum levels of the neutralizing antibodies (NAs) specific to the receptor-binding domain of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. Therefore, a high-throughput rapid assay capable of measuring the total SARS-CoV-2 NA level is urgently needed for COVID-19 serodiagnosis, convalescent plasma therapy, vaccine development, and assessment. Here, we developed a novel nanoplasmonic immunosorbent assay (NanoPISA) platform for one-step rapid quantification of SARS-CoV-2 NAs in clinical serum samples for high-throughput evaluation of COVID-19 vaccine effectiveness. The NanoPISA platform enhanced by the use of nanoporous hollow gold nanoparticle coupling was able to detect SARS-CoV-2 NAs with a limit of detection of 0.2 pM within 15 min without washing steps. The one-step NanoPISA for SARS-CoV-2 NA detection in clinical specimens yielded good results, comparable with those obtained in the gold-standard seroneutralization test and the surrogate virus-neutralizing enzyme-linked immunosorbent assay. Collectively, the one-step NanoPISA might be a rapid and high-throughput NA-quantification platform for evaluating the effectiveness of COVID-19 vaccines.
Keywords: COVID-19; Gold nanoparticles; Nanoplasmonic sensor; SARS-CoV-2 neutralizing antibody; SARS-CoV-2 variant.
【저자키워드】 COVID-19, SARS-CoV-2 variant, Gold nanoparticles, Nanoplasmonic sensor, SARS-CoV-2 neutralizing antibody, 【초록키워드】 neutralizing antibody, SARS-CoV-2, Vaccine development, coronavirus, COVID-19 vaccine, Neutralizing antibodies, SARS-CoV-2 variant, severe acute respiratory syndrome Coronavirus, Spike protein, enzyme-linked immunosorbent assay, COVID-19 vaccines, Receptor-binding domain, Convalescent plasma therapy, limit of detection, Effectiveness, gold, Neutralizing, respiratory, quantification, platform, SARS-CoV-2 neutralizing antibody, Gold nanoparticle, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, Serum level, Na level, vaccination efficacy, serum levels, specimen, serum sample, enzyme-linked immunosorbent, detect, the receptor-binding domain, comparable, virus-neutralizing, immunosorbent, 【제목키워드】 COVID-19 vaccination, novel, quantification, platform, Serum neutralizing antibody,