Abstract
Background: Serology assays have the potential to support RT-PCR in the diagnosis of SARS-CoV-2 infection. We studied three commercially available immunoassays for their diagnostic accuracy from blood specimens collected from 93 patients.
Methods: Blood samples from patients with confirmed COVID-19 infection were analysed using three different Immunoassays (Roche total antibody assay, Abbott IgG assay and Euroimmun IgG assay). Sensitivity, specificity, precision and time of seroconversion were evaluated.
Results: The sensitivity of Roche, Abbott and Euroimmun assays was 38.7%, 35.5% and 25.0% respectively for specimens collected <10 days and 84.4%, 84.4% and 70.0% respectively for specimens collected ≥10 days after the first positive RT-PCR. The specificity of all the three assays in this study was 100%. The timing of seroconversion occurred at day 1, 7 or 14.
Conclusions: The assays evaluated in this study have different sensitivities for detecting antibodies in SARS-CoV-2 infection. Sensitivity for detecting antibodies for all three assays was higher for specimens collected ≥10 days after first positive PCR compared with specimens collected <10 days. Time of seroconversion is variable and assay-dependent.
Keywords: COVID-19; SARS-CoV-2; Seroconversion; Serology.
【저자키워드】 COVID-19, SARS-CoV-2, Serology., Seroconversion, 【초록키워드】 IgG, serology, antibody, SARS-COV-2 infection, Infection, diagnostic, RT-PCR, immunoassays, immunoassay, sensitivity, specificity, Diagnostic accuracy, Seroconversion, PCR, Accuracy, COVID-19 infection, Patient, Roche, Euroimmun, patients, Blood, Abbott, blood specimens, Support, Precision, Blood samples, blood sample, specimen, Confirmed COVID-19 infection, serology assays, time, first positive, collected, occurred, evaluated, analysed, diagnosis of SARS-CoV-2, 【제목키워드】 hospital, Care, serology assay, United Arab Emirate,