Abstract
Knowledge on the immunogenicity of vector-based and mRNA-vaccines in solid organ transplant recipients is limited. Therefore, SARS-CoV-2-specific T cells and antibodies were analyzed in 40 transplant recipients and 70 controls after homologous or heterologous vaccine-regimens. Plasmablasts and SARS-CoV-2-specific CD4 and CD8 T cells were quantified using flow cytometry. Specific antibodies were analyzed by ELISA and neutralization assay. The two vaccine types differed after the first vaccination, as IgG and neutralizing activity were more pronounced after mRNA priming (p = .0001 each), whereas CD4 and CD8 T cell levels were higher after vector priming (p = .009; p = .0001). All regimens were well tolerated, and SARS-CoV-2-specific antibodies and/or T cells after second vaccination were induced in 100% of controls and 70.6% of transplant recipients. Although antibody and T cell levels were lower in patients, heterologous vaccination led to the most pronounced induction of antibodies and CD4 T cells. Plasmablast numbers were significantly higher in controls and correlated with SARS-CoV-2-specific IgG- and T cell levels. While antibodies were only detected in 35.3% of patients, cellular immunity was more frequently found (64.7%) indicating that assessment of antibodies is insufficient to identify COVID-19-vaccine responders. In conclusion, heterologous vaccination seems promising in transplant recipients, and combined analysis of humoral and cellular immunity improves the identification of responders among immunocompromised individuals.
Keywords: T cell biology; clinical research/practice; flow cytometry; infection and infectious agents – viral; infectious disease; vaccine.
【저자키워드】 Infectious disease, flow cytometry, vaccine., T cell biology, clinical research/practice, infection and infectious agents - viral, 【초록키워드】 IgG, Vaccine, vaccination, immunogenicity, antibody, T cells, Infection, Infectious disease, CD4, flow cytometry, ELISA, Cell Biology, Neutralizing activity, T cell, Neutralization assay, cellular immunity, mRNA, plasmablasts, Control, Responder, homologous, SARS-CoV-2-specific antibodies, patients, CD8 T cells, Heterologous, SARS-CoV-2-specific T cells, humoral and cellular immunity, solid organ transplant, specific antibodies, CD4 T cells, Analysis, heterologous vaccine, CD8 T cell, solid organ, humoral, regimen, infectious agents, infectious agent, SARS-CoV-2-specific antibody, immunocompromised individuals, recipients, second vaccination, regimens, plasmablast, responders, priming, CD4 and CD8 T cells, SARS-CoV-2-specific IgG, first vaccination, recipient, Specific, while, mRNA-vaccine, mRNA-vaccines, IMPROVE, analyzed, identify, correlated, significantly higher, homologous or heterologous, quantified, SARS-CoV-2-specific T cell, 【제목키워드】 COVID-19 vaccine, Immunity, Humoral immunity, mRNA, Heterologous, solid organ transplant, regimen, recipient,