Abstract
Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS, a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy chains. Ig-MS uses recent advances in protein mass spectrometry (MS) for multiparametric readout of antibodies, with new metrics like Ion Titer (IT) and Degree of Clonality (DoC) capturing the heterogeneity and relative abundance of individual clones without sequencing of B cells. We applied Ig-MS to plasma from subjects with severe and mild COVID-19 and immunized subjects after two vaccine doses, using the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 as the bait for antibody capture. Importantly, we report a new data type for human serology, that could use other antigens of interest to gauge immune responses to vaccination, pathogens, or autoimmune disorders.
Keywords: COVID-19; SARS-CoV-2; antibodies; individual ion mass spectrometry; proteomics; serology; top-down mass spectrometry.
【저자키워드】 COVID-19, antibodies, SARS-CoV-2, proteomics, serology, individual ion mass spectrometry, top-down mass spectrometry., 【초록키워드】 proteomics, mass spectrometry, immune response, vaccination, vaccine doses, Immunity, serology, antibody, Sequencing, B cells, heterogeneity, Spike protein, Antigen, Protein, Region, Antibody detection, Receptor-binding domain, pathogen, Immunoglobulin, RBD, titer, immune responses, Mild, plasma, Pathogens, molecular, serological, Autoimmune disorders, capture, variable regions, captures, Metrics, data type, heavy chains, subject, clone, degree, immunized, Clonality, applied, the spike protein, the receptor-binding domain, Ion, 【제목키워드】 mass, repertoire, the SARS-CoV-2,