Abstract
The COVID-19 pandemic has resulted in a worldwide health crisis. Rapid diagnosis, new therapeutics and effective vaccines will all be required to stop the spread of COVID-19. Quantitative evaluation of serum antibody levels against the SARS-CoV-2 virus provides a means of monitoring a patient’s immune response to a natural viral infection or vaccination, as well as evidence of a prior infection. In this paper, a portable and low-cost electrochemical immunosensor is developed for the rapid and accurate quantification of SARS-CoV-2 serum antibodies. The immunosensor is capable of quantifying the concentrations of immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies against the SARS-CoV-2 spike protein in human serum. For IgG and IgM, it provides measurements in the range of 10.1 ng/mL – 60 μg/mL and 1.64 ng/mL – 50 μg/mL, respectively, both with an assay time of 13 min. We also developed device stabilization and storage strategies to achieve stable performance of the immunosensor over 24-week storage at room temperature. We evaluated the performance of the immunosensor using COVID-19 patient serum samples collected at different time points after symptom onset. The rapid and sensitive detection of IgG and IgM provided by our immunosensor fulfills the need of rapid COVID-19 serological testing for both point-of-care diagnosis and population immunity screening.
Keywords: COVID-19; Electrochemical biosensing; Immunity screening; Point-of-care diagnosis; Serological test.
【저자키워드】 COVID-19, electrochemical biosensing, Immunity screening, Point-of-care diagnosis, Serological test., 【초록키워드】 serological test, SARS-CoV-2, IgG, IgM, viral infection, immune response, vaccination, antibody, COVID-19 pandemic, Infection, Diagnosis, SARS-CoV-2 virus, Spike protein, Health crisis, point-of-care, Immunoglobulin G, Health, Viral, Immunoglobulin, Serological testing, SARS-CoV-2 spike protein, Patient, Rapid, temperature, serum antibody, population immunity, quantification, human serum, Evidence, Concentration, immunoglobulin M, IgG and IgM, evidence of, room temperature, symptom onset, serum samples, different time points, Effective vaccines, serum antibodies, COVID-19 patient serum, spread of COVID-19, collected, evaluated, required, provided, provide, effective vaccine, different time point, the SARS-CoV-2, the SARS-CoV-2 virus, 【제목키워드】 SARS-CoV-2 antibody, platform,