Abstract
Enzyme-linked immunosorbent assay is widely utilized in serologic assays, including COVID-19, for the detection and quantification of antibodies against SARS-CoV-2. However, due to the limited stability of the diagnostic reagents (e.g., antigens serving as biorecognition elements) and biospecimens, temperature-controlled storage and handling conditions are critical. This limitation among others makes biodiagnostics in resource-limited settings, where refrigeration and electricity are inaccessible or unreliable, particularly challenging. In this work, metal-organic framework encapsulation is demonstrated as a simple and effective method to preserve the conformational epitopes of antigens immobilized on microtiter plate under non-refrigerated storage conditions. It is demonstrated that in situ growth of zeolitic imidazolate framework-90 (ZIF-90) renders excellent stability to surface-bound SARS-CoV-2 antigens, thereby maintaining the assay performance under elevated temperature (40 °C) for up to 4 weeks. As a complementary method, the preservation of plasma samples from COVID-19 patients using ZIF-90 encapsulation is also demonstrated. The energy-efficient approach demonstrated here will not only alleviate the financial burden associated with cold-chain transportation, but also improve the disease surveillance in resource-limited settings with more reliable clinical data.
Keywords: SARS-CoV-2; enzyme-linked immunosorbent assays; metal-organic frameworks; preservation; resource-limited settings.
【저자키워드】 SARS-CoV-2, enzyme-linked immunosorbent assays, metal-organic frameworks, preservation, resource-limited settings., 【초록키워드】 COVID-19, antibody, Antigen, enzyme-linked immunosorbent assay, Epitopes, stability, Surveillance, enzyme-linked immunosorbent assays, antigens, temperature, Disease surveillance, encapsulation, epitope, serologic assays, quantification, Critical, COVID-19 patients, preservation, conformational epitopes, SARS-CoV-2 antigens, COVID-19 patient, complementary, elements, among others, Clinical data, enzyme, growth, plasma samples, biospecimens, handling, elevated temperature, storage conditions, refrigeration, electricity, approach, effective, enzyme-linked immunosorbent, diagnostic reagents, plasma sample, IMPROVE, the disease, condition, demonstrated, conformational, alleviate, diagnostic reagent, inaccessible, 【제목키워드】 COVID-19, framework, Enhancing,