Abstract
We aimed to describe the SARS-CoV-2 lineages circulating early pandemic among samples with S gene dropout and characterize the receptor-binding domain (RBD) of viral spike protein. Adults and children older than 2 months with signs and symptoms of COVID-19 were prospectively enrolled from May to October in Porto Alegre, Brazil. All participants performed RT-PCR assay, and samples with S gene dropout and cycle threshold < 30 were submitted to high-throughput sequencing (HTS). 484 out of 1,557 participants tested positive for SARS-CoV-2. The S gene dropout was detected in 7.4% (36/484) and a peak was observed in August. The B.1.1.28, B.1.91 and B.1.1.33 lineages were circulating in early pandemic. The RBD novel mutation (Y380Q) was found in one sample occurring simultaneously with C379W and V395A, and the B.1.91 lineage in the spike protein. The Y380Q and C379W may interfere with the binding of neutralizing antibodies (CR3022, EY6A, H014, S304).
Keywords: COVID-19; Novel mutation; RBD; SARS-CoV-2; Variants.
【저자키워드】 COVID-19, SARS-CoV-2, RBD, variants., Novel mutation, 【초록키워드】 neutralizing antibody, Brazil, pandemic, Mutation, Neutralizing antibodies, children, Sequencing, variants, Spike protein, Viral, Receptor-binding domain, cycle threshold, Signs and symptoms, Lineage, High-throughput sequencing, B.1.1.28, novel, RT-PCR assay, binding, S gene, CR3022, viral spike protein, Older, All participants, participant, circulating, symptoms of COVID-19, Porto, positive, B.1.1.33, EY6A, enrolled, tested, performed, the spike protein, the receptor-binding domain, interfere, submitted, All participant, the SARS-CoV-2, 【제목키워드】 neutralizing antibody, Mutation, SARS-CoV-2 spike protein, Interaction, domain, interfere,