Abstract
The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic has once more emphasized the urgent need for accurate and fast point-of-care (POC) diagnostics for outbreak control and prevention. The main challenge in the development of POC in vitro diagnostics (IVD) is to combine a short time to result with a high sensitivity, and to keep the testing cost-effective. In this respect, sensors based on photonic integrated circuits (PICs) may offer advantages as they have features such as a high analytical sensitivity, capability for multiplexing, ease of miniaturization, and the potential for high-volume manufacturing. One special type of PIC sensor is the asymmetric Mach-Zehnder Interferometer (aMZI), which is characterized by a high and tunable analytical sensitivity. The current work describes the application of an aMZI-based biosensor platform for sensitive and multiplex detection of anti-SARS-CoV-2 antibodies in human plasma samples using the spike protein (SP), the receptor-binding domain (RBD), and the nucleocapsid protein (NP) as target antigens. The results are in good agreement with several CE-IVD marked reference methods and demonstrate the potential of the aMZI biosensor technology for further development into a photonic IVD platform.
Keywords: SARS-CoV-2; antibodies; biosensor; diagnostics; photonics; serology.
【저자키워드】 antibodies, SARS-CoV-2, serology, diagnostics, biosensor, photonics, 【초록키워드】 severe acute respiratory syndrome coronavirus 2, coronavirus, pandemic, serology, POC, Multiplexing, in vitro, severe acute respiratory syndrome Coronavirus, coronavirus 2, Spike protein, nucleocapsid protein, biosensor, Severe acute respiratory syndrome, point-of-care, sensitivity, anti-SARS-CoV-2 antibodies, Receptor-binding domain, anti-SARS-CoV-2 antibody, outbreak, RBD, antigens, respiratory, multiplex, platform, photonics, analytical sensitivity, miniaturization, acute respiratory syndrome coronavirus, reference method, offer, asymmetric, IVD, feature, human plasma samples, characterized, the spike protein, the receptor-binding domain, human plasma sample, Interferometer, 【제목키워드】 Human, detection, sensitive,