Abstract
A lateral flow assay (LFA) platform is a powerful tool for point-of-care testing (POCT), especially for self-testing. Although the LFA platform provides a simple and disposable tool for Coronavirus disease of 2019 (COVID-19) antigen (Ag) and antibody (Ab) screening tests, the lower sensitivity for low virus titers has been a bottleneck for practical applications. Herein, we report the combination of a microfluidic paper-based nanoelectrokinetic (NEK) preconcentrator and an LFA platform for enhancing the sensitivity and limit of detection (LOD). Biomarkers were electrokinetically preconcentrated onto a specific layer using the NEK preconcentrator, which was then coupled with LFA diagnostic devices for enhanced performance. Using this nanoelectrokinetic-assisted LFA (NEK-LFA) platform for self-testing, the severe acute respiratory syndrome coronavirus 2 Immunoglobulin G (SARS-CoV-2 IgG) sample was preconcentrated from serum samples. After preconcentration, the LOD of the LFA was enhanced by 32-fold, with an increase in analytical sensitivity (16.4%), which may offer a new opportunity for POCT and self-testing, especially in the COVID-19 pandemic and endemic global context.
Keywords: Biomarker; COVID-19; Lateral flow assay; Preconcentration; SARS-CoV-2 IgG; Self-testing.
【저자키워드】 COVID-19, Biomarker, lateral flow assay, SARS-CoV-2 IgG, Preconcentration, Self-testing., 【초록키워드】 coronavirus disease, SARS-CoV-2, coronavirus, Biomarker, Biomarkers, antibody, COVID-19 pandemic, diagnostic, severe acute respiratory syndrome Coronavirus, Antigen, lateral flow, point-of-care, sensitivity, Immunoglobulin G, Immunoglobulin, limit of detection, POCT, respiratory, disease, platform, SARS-CoV-2 IgG, 2019, analytical sensitivity, Endemic, Combination, screening tests, virus titers, virus titer, acute respiratory syndrome, serum samples, powerful tool, offer, provide, increase in, Lateral, 【제목키워드】 lateral flow, COVID-19 antibody test,