Abstract
A recently identified SARS-CoV-2 variant, Lambda, has spread to many countries around the world. Here, we measured and evaluated the reduced sensitivity of Lambda variant to the neutralization by plasma polyclonal antibodies elicited by the natural SARS-CoV-2 infection and inactivated vaccine. The combination of two substitutions appearing in the RBD of spike protein (L452Q and F490S) resulted in noticeably reduced neutralization against Lambda variant. F490S contributed more than L452Q in affecting the neutralization. In addition, the neutralization test with 12 published nAbs binding to RBD of SARS-CoV-2 with defined structures suggested that Lambda variant resisted the neutralization by some antibodies from Class 2 and Class 3. Overall, these results suggest that pre-existing antibody neutralization established by natural infection from non-Lambda variants or immunization could be significantly decreased, re-emphasizing the importance of ongoing viral mutation monitoring.
Keywords: Convalescent plasma; Inactivated vaccine; Lambda variant; Monoclonal antibody; Neutralization; SARS-CoV-2.
【저자키워드】 convalescent plasma, SARS-CoV-2, neutralization, monoclonal antibody, Inactivated vaccine, Lambda variant, 【초록키워드】 convalescent plasma, Structure, SARS-CoV-2, antibody, neutralization, SARS-COV-2 infection, monoclonal antibody, variant, SARS-CoV-2 variant, immunization, Spike protein, sensitivity, Viral, Inactivated vaccine, neutralization test, plasma, polyclonal antibodies, natural infection, Antibody neutralization, NAb, Lambda, binding, Combination, polyclonal antibody, Substitution, class, viral mutation, RBD of SARS-CoV-2, country, defined, spread to, significantly, addition, evaluated, reduced, suggested, the RBD, contributed, elicited, affecting, 【제목키워드】 SARS-CoV-2, variant, Antibody neutralization, Increased,