Abstract
An easily implementable serological assay to accurately detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibodies is urgently needed to better track herd immunity, vaccine efficacy and vaccination rates. Herein, we report the Split-Oligonucleotide Neighboring Inhibition Assay (SONIA) which uses real-time qPCR to measure the ability of neutralizing antibodies to block binding between DNA-barcoded viral spike protein subunit 1 and the human angiotensin-converting enzyme 2 receptor protein. The SONIA neutralizing antibody assay using finger-prick dried blood spots displays 91-97% sensitivity and 100% specificity in comparison to the live-virus neutralization assays using matched serum specimens for multiple SARS-CoV-2 variants-of-concern. The multiplex version of this neutralizing antibody assay, using easily collectable finger-prick dried blood spots, can be a valuable tool to help reveal the impact of age, pre-existing health conditions, waning immunity, different vaccination schemes and the emergence of new variants-of-concern.
【초록키워드】 neutralizing antibody, SARS-CoV-2, Efficacy, Vaccine, coronavirus, vaccination, Immunity, Neutralizing antibodies, vaccine efficacy, inhibition, severe acute respiratory syndrome Coronavirus, Spike protein, serum, sensitivity, specificity, Dried blood spots, dried blood spot, Health, Viral, Serological assay, Neutralization assay, herd immunity, age, respiratory, multiplex, binding, angiotensin, waning immunity, viral spike protein, acute respiratory syndrome, subunit, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, receptor protein, specimen, human Angiotensin-converting enzyme, help, vaccination rates, real-time qPCR, detect, conditions, 【제목키워드】 neutralizing antibody, detection, dried blood spot, PCR, SARS-CoV-2 strain,