Abstract
SARS-CoV-2 infection is a global public health threat. Vaccines are considered amongst the most important tools to control the SARS-CoV-2 pandemic. As expected, deaths from SARS-CoV-2 infection have dropped dramatically with widespread vaccination. However, there are concerns over the duration of vaccine-induced protection, as well as their effectiveness against emerging variants of concern. Here, we constructed a recombinant chimpanzee adenovirus vectored vaccine expressing the full-length spike of SARS-CoV-2 (AdC68-S). Rapid and high levels of humoral and cellular immune responses were observed after immunization of C57BL/6J mice with one or two doses of AdC68-S. Notably, neutralizing antibodies were observed up to at least six months after vaccination, without substantial decline. Single or double doses AdC68-S immunization resulted in lower viral loads in lungs of mice against SARS-CoV-2 challenge both in the short term (21 days) and long-term (6 months). Histopathological examination of AdC68-S immunized mice lungs showed mild histological abnormalities after SARS-CoV-2 infection. Taken together, this study demonstrates the efficacy and durability of the AdC68-S vaccine and constitutes a promising candidate for clinical evaluation.
Keywords: Chimpanzee adenovirus vector; Long-term protection; Neutralizing antibodies; SARS-CoV-2; Vaccine.
【저자키워드】 SARS-CoV-2, Neutralizing antibodies, vaccine., Chimpanzee adenovirus vector, Long-term protection, 【초록키워드】 neutralizing antibody, Efficacy, Vaccine, vaccination, pandemic, Neutralizing antibodies, Cellular immune response, SARS-COV-2 infection, SARS-CoV-2 pandemic, Infection, variants of concern, lung, immunization, Adenovirus, Viral, Viral load, clinical evaluation, mice, immune responses, Lungs, Effectiveness, death, Rapid, Mild, Humoral and cellular immune responses, dose, humoral, short term, global public health, vectored vaccine, histopathological examination, immunized mice, full-length, widespread, histological abnormalities, expressing, expected, Chimpanzee, dropped, histological abnormality, recombinant chimpanzee adenovirus, the SARS-CoV-2, 【제목키워드】 Vaccine, recombinant, effective, provide, the spike protein, expressing,