Abstract
Rapid antigen detection tests are urgently needed for the early diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The discovery of a binder with high affinity and selectivity for the biomarkers presented by SARS-CoV-2 is crucial to the development of the rapid antigen detection method. We utilized the surface biopanning to identify a peptide binder R1 from a phage-displayed peptide library consisting of 10 9 independent phage recombinants. The R1 peptide exhibited high-affinity for specific binding with the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein with a dissociation constant K D of (7.5 ± 1.9) × 10 -10 M, which maintained high binding affinity with the RBD derived from Gamma, Lambda, Delta, and Omicron variants. The composition and sequence dependence of binding characteristics in R1-RBD interactions was revealed by the binding affinity fluctuations between RBD and the scrambled sequences or single-site mutants of R1. The R1-functionalized gold nanoparticles possessed concentration-dependent response to RBD and selectivity over bovine serum albumin and human serum albumin. The peptide binder R1 shows the potential to be used for constructing a rapid detection method for the early-stage diagnostics for SARS-CoV-2.
Keywords: affinity peptide; assembly; interface; receptor-binding domain; sensor.
【저자키워드】 Receptor-binding domain, affinity peptide, assembly, interface, sensor., 【초록키워드】 SARS-CoV-2, coronavirus, Biomarker, Biomarkers, Delta, peptide, severe acute respiratory syndrome Coronavirus, omicron, Omicron variants, diagnostics, variants, binding affinity, Spike protein, early diagnosis, sensor, Receptor-binding domain, Characteristics, RBD, SARS-CoV-2 spike protein, albumin, Rapid, Gamma, gold, bovine, mutant, selectivity, serum albumin, respiratory, antigen detection, dissociation, Lambda, assembly, interface, binding, Gold nanoparticle, Dissociation constant, Interaction, acute respiratory syndrome, Human serum albumin, bovine serum albumin, rapid detection method, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, sequence, high affinity, phage, recombinants, independent, binder, fluctuation, identify, exhibited, the RBD, the receptor-binding domain, the binding affinity, the SARS-CoV-2, 【제목키워드】 spike, the SARS-CoV-2,