Abstract
Serological detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N), spike (S), and neutralizing antibodies (Abs) is commonly undertaken to evaluate the efficacy of vaccination. However, the relative efficiency of different SARS-CoV-2 Ab detection systems has not been extensively investigated. Here, we evaluated serological test systems in vaccinated Japanese. SARS-CoV-2 N, S, and neutralizing Abs in sera of 375 healthy subjects a mean 253 days after vaccination were assessed. The sensitivity of Elecsys Anti-SARS-CoV-2 S (Roche S) and Anti-SARS-CoV-2 S IgG (Fujirebio S) was 100% and 98.9%, respectively, with a specificity of 100% for both. The sensitivity of Anti-SARS-CoV-2 neutralizing Ab (MBL Neu) was 2.7%, and the specificity was 100%. Fujirebio S correlated with Roche S (rho = 0.9182, p = 3.97 × 10 -152 ). Fujirebio S (rho = 0.1295, p = 0.0121) and Roche S (rho = 0.1232, p = 0.0170) correlated weakly with MBL Neu. However, Roche S did correlate with MBL Neu in patients with COVID-19 (rho = 0.8299, p = 1.01 × 10 -12 ) and in healthy subjects more recently after vaccination (mean of 90 days, rho = 0.5306, p = 0.0003). Thus, the Fujirebio S and Roche S results were very similar, but neither correlated with neutralizing antibody titers by MBL Neu at a later time after vaccination.
Keywords: SARS-CoV-2 antibody; neutralizing antibody; nucleocapsid; spike; vaccination.
【저자키워드】 neutralizing antibody, spike, vaccination., SARS-CoV-2 antibody, nucleocapsid, 【초록키워드】 neutralizing antibody, serological test, SARS-CoV-2, IgG, Efficacy, coronavirus, vaccination, Neutralizing antibodies, antibody, severe acute respiratory syndrome Coronavirus, sensitivity, specificity, SARS-CoV-2 antibody, nucleocapsid, sera, Neutralizing, Japanese, respiratory, Roche, Antibody titers, Neutralizing antibody titer, Efficiency, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, healthy subjects, neutralizing antibody titers, Fujirebio, Elecsys, SARS-CoV-2 N, detection system, evaluate, investigated, evaluated, correlated, healthy subject, Elecsy, patients with COVID-19, were assessed, 【제목키워드】 SARS-CoV-2, detection, Japanese,