Abstract
Background: Specialized proresolution molecules (SPMs) halt the transition to chronic pathogenic inflammation. We aimed to quantify serum levels of pro- and anti-inflammatory bioactive lipids in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) patients, and to identify potential relationships with innate responses and clinical outcome.
Methods: Serum from 50 hospital admitted inpatients (22 female, 28 male) with confirmed symptomatic SARS-CoV-2 infection and 94 age- and sex-matched controls collected prior to the pandemic (SARS-CoV-2 negative), were processed for quantification of bioactive lipids and anti-nucleocapsid and anti-spike quantitative binding assays.
Results: SARS-CoV-2 serum had significantly higher concentrations of omega-6-derived proinflammatory lipids and omega-6- and omega-3-derived SPMs, compared to the age- and sex-matched SARS-CoV-2-negative group, which were not markedly altered by age or sex. There were significant positive correlations between SPMs, proinflammatory bioactive lipids, and anti-spike antibody binding. Levels of some SPMs were significantly higher in patients with an anti-spike antibody value >0.5. Levels of linoleic acid and 5,6-dihydroxy-8Z,11Z,14Z-eicosatrienoic acid were significantly lower in SARS-CoV-2 patients who died.
Conclusions: SARS-CoV-2 infection was associated with increased levels of SPMs and other pro- and anti-inflammatory bioactive lipids, supporting the future investigation of the underlying enzymatic pathways, which may inform the development of novel treatments.
Keywords: SARS-CoV-2; anti-nucleocapsid; bioactive lipids; immune response; specialized proresolving molecules.
【저자키워드】 SARS-CoV-2, immune response, Anti-nucleocapsid, bioactive lipids, specialized proresolving molecules., 【초록키워드】 coronavirus, immune response, pandemic, Anti-inflammatory, SARS-COV-2 infection, hospital, Sex, severe acute respiratory syndrome Coronavirus, immune, Clinical outcome, proinflammatory, serum, male, female, Patient, Control, age, respiratory, lipids, quantification, Quantitative, patients, Anti-spike, anti-spike antibody, binding, SARS-CoV-2 patients, Concentration, Lipid, Pathways, linoleic acid, Inpatient, innate response, acute respiratory syndrome, Omega, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, Serum level, serum levels, transition, positive correlation, significantly lower, symptomatic SARS-CoV-2 infection, pathogenic inflammation, identify, collected, died, assays, significantly higher, Level, processed, SARS-CoV-2 patient, 【제목키워드】 coronavirus 2, response, Lipid, mediator, molecule, Level, Increased,