Abstract
An outbreak of coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses great threats to human health and the international economy. To reduce large-scale infection and transmission risk of SARS-CoV-2, a simple, rapid, and sensitive serological diagnostic method is urgently needed. Herein, an aggregation-induced emission (AIE) nanoparticle (AIE 810 NP, λ em = 810 nm)-labeled lateral flow immunoassay was designed for early detection of immunoglobulin M (IgM) and immunoglobulin G (IgG) against SARS-CoV-2 in clinical serum samples. Using a near-infrared (NIR) AIE nanoparticle as the fluorescent reporter (△λ = 145 nm), the autofluorescence from the nitrocellulose membrane and biosample and the excitation background noise were effectively eliminated. After optimization, the limit of detection of IgM and IgG is 0.236 and 0.125 μg mL -1 , respectively, commensurate with that of the enzyme-linked immunosorbent assay (ELISA) (0.040 and 0.039 μg mL -1 ). The sensitivity of the proposed AIE 810 NP-based test strip for detecting IgM and IgG is 78 and 95% (172 serum samples), commensurate with that of ELISA (85 and 95%) and better than that of a commercial colloidal gold nanoparticle (AuNP)-based test strip (41 and 85%). Importantly, the time of detecting IgM or IgG with an AIE 810 NP-based test strip in sequential clinical samples is 1-7 days after symptom onset, which is significantly earlier than that with a AuNP-based test strip (8-15 days). Therefore, the NIR-emissive AIE nanoparticle-labeled lateral flow immunoassay holds great potential for early detection of IgM and IgG in a seroconversion window period.
Keywords: COVID-19; NIR-emissive AIE nanoparticle; SARS-CoV-2; early detection; lateral flow immunoassay.
【저자키워드】 COVID-19, SARS-CoV-2, lateral flow immunoassay, Early detection, NIR-emissive AIE nanoparticle, 【초록키워드】 coronavirus disease, IgG, IgM, coronavirus, threat, Infection, risk, Transmission, severe acute respiratory syndrome Coronavirus, ELISA, lateral flow immunoassay, sensitivity, enzyme-linked immunosorbent assay, Immunoglobulin G, Health, Seroconversion, Immunoglobulin, outbreak, International, Early detection, limit of detection, gold, respiratory, reporter, serological, optimization, Diagnostic method, Gold nanoparticle, immunoglobulin M, IgM and IgG, colloidal gold, nitrocellulose, test strip, background noise, symptom onset, acute respiratory syndrome, Nitrocellulose membrane, acute respiratory syndrome coronavirus, serum samples, acute respiratory syndrome coronavirus 2, enzyme, fluorescent, AuNP, enzyme-linked immunosorbent, autofluorescence, clinical sample, caused, significantly, reduce, eliminated, 【제목키워드】 Human, detection, Early detection, Flow, emission, Lateral,