Abstract
Combined kinetic analysis of plasma SARS-CoV-2 RNAemia, Nucleocapsid (N)-antigenemia and virus-specific antibodies may help ascertain the role of antibodies in preventing virus dissemination in COVID-19 patients. We performed this analysis in a cohort of 71 consecutive critically ill COVID-19 patients (49 male; median age, 65 years) using RT-PCR assay, lateral flow immunochromatography method and receptor binding domain (RBD) and N-based immunoassays. A total of 338 plasma specimens collected at a median of 12 days after symptoms onset were available for analyses. SARS-CoV-2 RNAemia and N-antigenemia were detected in 37 and 43 specimens from 26 (36.5%) and 30 (42.2%) patients, respectively. Free RNA was the main biological form of SARS-CoV-2 found in plasma. The detection rate for both viral components was associated with viral load at the upper respiratory tract. Median time to SARS-CoV-2-RBD antibody detection was 14 days (range, 4-38) from onset of symptoms. Decreasing antibody levels were observed in parallel to increasing levels of both RNAemia and N-antigenemia, yet overall a fairly modest inverse correlation (Rho = -0.35; P < 0.001) was seen between virus RNAemia and SARS-CoV-2-RBD antibody levels. The data cast doubts on a major involvement of antibodies in virus clearance from the bloodstream within the timeframe examined.
【초록키워드】 immunochromatography, SARS-CoV-2, antibody, virus, Receptor binding domain, RNA, lateral flow, immunoassays, Cohort, Viral, Antibody detection, Critically ill, Viral load, nucleocapsid, RBD, antigenemia, plasma, respiratory, dissemination, upper respiratory tract, detection rate, RT-PCR assay, patients, COVID-19 patients, specific antibodies, Analysis, COVID-19 patient, median time, virus clearance, median age, specimen, bloodstream, help, symptoms onset, CAST, inverse correlation, component, virus-specific antibodies, SARS-CoV-2-RBD, kinetic analysis, Combined, Free, decreasing, performed, collected, examined, median, onset of symptoms, analyses, 【제목키워드】 SARS-CoV-2, antibody, kinetic analysis, Combined, adult COVID-19 patient,