Abstract
Objectives: The current study was aimed at examining SARS-CoV-2 immune responses following two doses of Comirnaty® COVID-19 vaccine among elderly people in nursing homes.
Methods: A prospective cohort study in a representative sample from nursing homes in Valencia (n = 881; males: 271, females 610; median age, 86 years) recruited residents using a random one-stage cluster sampling approach. A lateral flow immunochromatography device (LFIC) (OnSite COVID-19 IgG/IgM Rapid Test; CTK BIOTECH, Poway, CA, USA) was used as the front-line test for detecting SARS-CoV-2-Spike (S)-specific antibodies in whole blood obtained using a fingerstick. Residents returning negative LFIC results underwent venipuncture and testing for presence of SARS-CoV-2-S-reactive antibodies and T cells using the Roche Elecsys® Anti-SARS-CoV-2 S (Roche Diagnostics, Pleasanton, CA, USA), the LIAISON® SARS-CoV-2 TrimericS IgG assay (Diasorin S.p.A, Saluggia, Italy) and by flow cytometry, respectively.
Results: The SARS-CoV-2-S antibody detection rate in nursing home residents was 99.6% (283/284) and 98.3% (587/597) for SARS-CoV-2 recovered and naïve residents, respectively, within a median of 99 days (range 17-125 days) after full vaccination. Three out of five residents lacking SARS-CoV-2-S antibodies had detectable S-reactive CD8 + and/or CD4 + T cells. In addition, 50/50 and 40/50 participants with detectable SARS-CoV-2 antibodies also had SARS-CoV-2-S-reactive interferon-γ-producing CD4 + and CD8 + T cells, respectively.
Discussion: The Comirnaty® COVID-19 vaccine is highly immunogenic in nursing home residents.
Keywords: Comirnaty®COVID-19 vaccine; Nursing home residents; SARS-CoV-2; SARS-CoV-2-S antibodies.
【저자키워드】 SARS-CoV-2, nursing home residents, Comirnaty®COVID-19 vaccine, SARS-CoV-2-S antibodies., 【초록키워드】 COVID-19, antibodies, immunochromatography, IgG, COVID-19 vaccine, immune response, vaccination, antibody, T cells, nursing home, interferon, Italy, prospective cohort study, CD4, CD8, resident, flow cytometry, lateral flow, T cell, SARS-CoV-2 antibodies, Antibody detection, SARS-CoV-2 antibody, Rapid test, Whole blood, immune responses, female, Rapid, Cluster, USA, nursing homes, Roche, specific antibodies, dose, median age, naïve, participant, immunogenic, random, Roche Diagnostics, fingerstick, venipuncture, SARS-CoV-2-S, Anti-SARS-CoV-2 S, approach, FIVE, Valencia, was used, recruited, addition, detectable, median, Elecsys® Anti-SARS-CoV-2 S, 【제목키워드】 administration,