Abstract
SARS-CoV-2 specific T-cell response has been associated with disease severity, immune memory and heterologous response to endemic coronaviruses. However, an integrative approach combining a comprehensive analysis of the quality of SARS-CoV-2 specific T-cell response with antibody levels in these three scenarios is needed. In the present study, we found that, in acute infection, while mild disease was associated with high T-cell polyfunctionality biased to IL-2 production and inversely correlated with anti-S IgG levels, combinations only including IFN-γ with the absence of perforin production predominated in severe disease. Seven months after infection, both non-hospitalised and previously hospitalised patients presented robust anti-S IgG levels and SARS-CoV-2 specific T-cell response. In addition, only previously hospitalised patients showed a T-cell exhaustion profile. Finally, combinations including IL-2 in response to S protein of endemic coronaviruses were the ones associated with SARS-CoV-2 S-specific T-cell response in pre-COVID-19 healthy donors’ samples. These results could have implications for protective immunity against SARS-CoV-2 and recurrent COVID-19 and may help for the design of new prototypes and boosting vaccine strategies.
Keywords: COVID-19; IL-2; SARS-CoV-2; Spike; T-cell response; endemic coronaviruses; nucleocapsid; polyfunctionality.
【저자키워드】 COVID-19, SARS-CoV-2, spike, T-cell Response, Endemic coronaviruses, nucleocapsid, IL-2, polyfunctionality, 【초록키워드】 Coronaviruses, Vaccine, coronavirus, S protein, antibody, disease severity, T-cell Response, Infection, protective immunity, nucleocapsid, acute infection, Mild, immune memory, antibody levels, T-cell, disease, T-cell exhaustion, Heterologous, IFN-γ, Mild disease, IL-2, anti-S IgG, Endemic, Combination, severe disease, comprehensive analysis, combinations, help, Specific T-cell response, perforin, anti-S IgG levels, implication, approach, healthy donor, robust, addition, absence, correlated, hospitalised patient, anti-S IgG level, non-hospitalised, 【제목키워드】 T-cell Response, Heterologous, Specific T-cell response,