Abstract
Here, we expressed two neutralizing monoclonal antibodies (Abs) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; H4 and B38) in three formats: IgG1, IgA1 monomers (m), and IgA1 dimers (d) in glycoengineered Nicotiana benthamiana plants. All six Ab variants assembled properly and exhibited a largely homogeneous glycosylation profile. Despite modest variation in antigen binding between Ab formats, SARS-CoV-2 neutralization (NT) potency significantly increased in the following manner: IgG1 < IgA1-m < IgA1-d, with an up to 240-fold NT increase of dimers compared to corresponding monomers. Our results underscore that both IgA’s structural features and multivalency positively impact NT potency. In addition, they emphasize the versatile use of plants for the rapid expression of complex human proteins.
Keywords: IgA; SARS-CoV-2; monoclonal antibodies; plant expression.
【저자키워드】 SARS-CoV-2, monoclonal antibodies, IgA, plant expression, 【초록키워드】 coronavirus, glycosylation, Variation, variant, severe acute respiratory syndrome Coronavirus, monoclonal antibodies, Antigen, plants, respiratory, plant, expression, IgG1, SARS-CoV-2 neutralization, neutralizing monoclonal antibodies, neutralizing monoclonal antibody, monoclonal, binding, Nicotiana benthamiana, acute respiratory syndrome, antigen binding, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, complex, human proteins, monomer, feature, significantly increased, addition, exhibited, expressed, homogeneous, 【제목키워드】 IgG, in vitro, Neutralizing activity, monomer, Increased,