Abstract
The novel coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented public health crisis and economic losses. Although several cases of cats and dogs infected with SARS-CoV-2 have been reported during this outbreak, the prevalence of SARS-CoV-2 in dog and its transmission among other companion animals are still unknown. Here, we report an extensive serological study of SARS-CoV-2 infection in dogs in Wuhan and analyse the infection rates at different stages of the pandemic outbreak. A total of 946 dogs serum samples were collected from Wuhan, of which 36 samples were obtained prior to the pandemic outbreak. Indirect enzyme-linked immunosorbent assay (ELISA) showed that 16 sera collected during the outbreak were detected as positive through the receptor-binding domain (RBD) of SARS-CoV-2. Of these 16 sera, 10 exhibited measurable SARS-CoV-2-specific neutralizing antibodies whose titres ranged from 1/20 to 1/180. No serological cross-reactivity was detected between SARS-CoV-2 and canine coronavirus (CCV). Furthermore, with the effective control of the outbreak, a decrease in the SARS-CoV-2 seropositive dog number was observed. Our results suggest that SARS-CoV-2 has infected companion dogs during the outbreak, and that COVID-19 patient families have a higher risk of dog infection. Our findings deepen our understanding of the infection of SARS-CoV-2 in dogs and provide an important reference for prevention of COVID-19.
Keywords: SARS-CoV-2; dog; neutralizing antibody; serological investigation.
【저자키워드】 neutralizing antibody, SARS-CoV-2, dog, serological investigation., 【초록키워드】 COVID-19, coronavirus disease, neutralizing antibody, coronavirus, pandemic, Neutralizing antibodies, SARS-COV-2 infection, Infection, Transmission, public health crisis, novel coronavirus disease, severe acute respiratory syndrome Coronavirus, ELISA, Health crisis, Novel coronavirus, cross-reactivity, Prevalence, enzyme-linked immunosorbent assay, Receptor-binding domain, cats, outbreak, RBD, sera, Wuhan, canine, Neutralizing, infection rate, respiratory, serological, seropositive, COVID-19 patient, acute respiratory syndrome, higher risk, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, titres, infection rates, Stage, titre, specific neutralizing antibodies, positive, serum sample, effective, decrease, enzyme-linked immunosorbent, prevention of COVID-19, collected, caused, reported, exhibited, the receptor-binding domain, ranged, CCV, infected with SARS-CoV-2, SARS-CoV-2-specific neutralizing antibody, the SARS-CoV-2, 【제목키워드】 acute respiratory syndrome, serological survey,