Abstract
Seven amino acid sequences of antibody mimetics molecules, monobodies, capable of interacting with the nucleocapsid protein of the SARS-CoV virus, were taken from the literature. Nucleotide sequences of monobody genes were obtained by gene synthesis, which were expressed in E. coli and isolated using Ni-NTA chromatography. It was shown by thermophoresis that three of the seven selected antibody-like molecules can interact with high affinity (dissociation constant of tens of nM) with the nucleocapsid protein of the SARS-CoV-2 virus. For the remaining four monobodies, only low affinity binding with a dissociation constant of several μM was found.
Keywords: SARS-CoV; SARS-CoV-2; antibody-like molecules; monobody; nucleocapsid protein; thermophoresis.
【저자키워드】 SARS-CoV-2, SARS-CoV, nucleocapsid protein, antibody-like molecules, monobody, thermophoresis, 【초록키워드】 antibody, SARS-CoV-2 virus, nucleocapsid protein, nucleocapsid, dissociation, monobody, Dissociation constant, Amino acid, nucleotide, amino acid sequence, E. coli, Amino acid sequences, sequence, high affinity, SARS-CoV virus, thermophoresis, affinity binding, Seven, selected, shown, were expressed, Ni-NTA, the SARS-CoV-2 virus, 【제목키워드】 molecules, affinity,