Abstract
The SARS-CoV-2 Delta (B.1.617.2) strain is a variant of concern (VOC) that has become the dominant strain worldwide in 2021. Its transmission capacity is approximately twice that of the original strain, with a shorter incubation period and higher viral load during infection. Importantly, the breakthrough infections of the Delta variant have continued to emerge in the first-generation vaccine recipients. There is thus an urgent need to develop a novel vaccine with SARS-CoV-2 variants as the major target. Here, receptor binding domain (RBD)-conjugated nanoparticle vaccines targeting the Delta variant, as well as the early and Beta/Gamma strains, are developed. Under both a single-dose and a prime-boost strategy, these RBD-conjugated nanoparticle vaccines induce the abundant neutralizing antibodies (NAbs) and significantly protect hACE2 mice from infection by the authentic SARS-CoV-2 Delta strain, as well as the early and Beta strains. Furthermore, the elicitation of the robust production of broader cross-protective NAbs against almost all the notable SARS-CoV-2 variants including the Omicron variant in rhesus macaques by the third re-boost with trivalent vaccines is found. These results suggest that RBD-based monovalent or multivalent nanoparticle vaccines provide a promising second-generation vaccine strategy for SARS-CoV-2 variants.
Keywords: Delta variants; SARS-CoV-2; nanoparticle vaccines.
【저자키워드】 SARS-CoV-2, Delta variants, nanoparticle vaccines., 【초록키워드】 neutralizing antibody, Vaccine, Vaccines, Neutralizing antibodies, VoC, variant, SARS-CoV-2 variant, Infection, Delta, B.1.617.2, Transmission, omicron, delta variant, hACE2, Receptor binding domain, Viral, SARS-CoV-2 variants, Viral load, mice, Incubation period, breakthrough infections, Omicron variant, Beta, Breakthrough infection, Strains, NAb, Delta variants, rhesus macaques, rhesus macaque, strain, recipients, NAbs, trivalent, SARS-CoV-2 delta, dominant, robust, PROTECT, develop, significantly, induce, cross-protective, notable, 【제목키워드】 Delta, nanoparticle, development, Other,