Abstract
Neutralizing antibody (NAb) detection is critical for evaluating herd immunity and monitoring the efficacy of vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, quantitative SARS-CoV-2 antibody levels after vaccination were measured by chemiluminescent immunoassays, enzyme immunoassays, and surrogate virus neutralization tests (sVNTs), as well as plaque reduction neutralization tests (PRNT). Sequential blood samples were collected before and 1 and 3 months after vaccination in 30 healthy participants (two doses of Oxford-AstraZeneca [AZ] or Pfizer-BioNTech [BNT]). After vaccination, all sera tested positive for PRNT, with NAb titers ranging from 1:10 to 1:723. Median NAb titers were higher in the BNT vaccine group than in the AZ vaccine group at both one and three months post-vaccination. Excellent overall concordance rates were observed between serological assays and PRNT. In a quantitative correlation analysis, the results of sVNTs showed a strong correlation with those of PRNT. Results of the four binding antibody assays showed a significant correlation with those of PRNT. The serologic assays evaluated in this study could be used as sVNTs to evaluate the efficacy of SARS-CoV-2 vaccines.
Keywords: ACE2-RBD inhibition assay; SARS-CoV-2; immunoassay; neutralizing antibody.
【저자키워드】 neutralizing antibody, SARS-CoV-2, immunoassay, ACE2-RBD inhibition assay, 【초록키워드】 neutralizing antibody, Efficacy, Vaccine, coronavirus, vaccination, Immunity, antibody, SARS-CoV-2 vaccines, severe acute respiratory syndrome Coronavirus, immunoassays, immunoassay, Serological assay, SARS-CoV-2 antibody, serological assays, herd immunity, sera, virus neutralization test, respiratory, correlation, serologic assays, Quantitative, Critical, Pfizer-BioNTech, PRNT, dose, Oxford-AstraZeneca, neutralization tests, binding antibody, enzyme immunoassays, acute respiratory syndrome, Correlation analysis, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, enzyme, sVNT, blood sample, participant, positive, NAb titers, ACE2-RBD, overall concordance, plaque reduction neutralization, vaccine group, AZ vaccine, NAb titer, serologic assay, Result, tested, collected, evaluate, healthy, evaluated, were measured, BNT, 【제목키워드】 detection, Population, Level,