Abstract
In this work, we report a facile synthesis of graphene oxide-gold (GO-Au) nanocomposites by electrodeposition. The fabricated electrochemical immunosensors are utilized for the dual detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen and SARS-CoV-2 antibody. The GO-Au nanocomposites has been characterized by UV-vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) for its biosensing properties. The linear detection range of the SARS-CoV-2 antigen immunosensor is 10.0 ag mL -1 to 50.0 ng mL -1 , whereas that for the antibody immunosensor ranges from 1.0 fg mL -1 to 1.0 ng mL -1 . The calculated limit of detection (LOD) of the SARS-CoV-2 antigen immunosensor is 3.99 ag mL -1 , and that for SARS-CoV-2 antibody immunosensor is 1.0 fg mL -1 with high sensitivity. The validation of the immunosensor has also been carried out on patient serum and patient swab samples from COVID-19 patients. The results suggest successful utilization of the immunosensors with a very low detection limit enabling its use in clinical samples. Further work is needed for the standardization of the results and translation in screen-printed electrodes for use in portable commercial applications.
Keywords: GO−Au nanocomposites; SARS-CoV-2 antibody; SARS-CoV-2 antigen; electrochemical immunosensor; electrodeposition.
【저자키워드】 SARS-CoV-2 antibody, SARS-CoV-2 antigen, electrochemical immunosensor, GO−Au nanocomposites, electrodeposition., 【초록키워드】 SARS-CoV-2, coronavirus, translation, antibody, severe acute respiratory syndrome Coronavirus, Antigen, clinical samples, serum, sensitivity, SARS-CoV-2 antibody, Patient, limit of detection, SARS-CoV-2 antigen, respiratory, transmission electron microscopy, COVID-19 patients, Detection limit, Standardization, dual, acute respiratory syndrome, X-ray diffraction, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, pulse, TEM, swab sample, carried, linear, characterized, calculated, DPV, the SARS-CoV-2, 【제목키워드】 SARS-CoV-2, detection, nanoparticle, gold, sensitive, Graphene, Oxide, nanocomposite,