Abstract
The immune response to SARS-CoV-2 infection requires antibody recognition of the spike protein. In a study designed to examine the molecular features of anti-spike and anti-nucleocapsid antibodies, patient plasma proteins binding to pre-fusion stabilised complete spike and nucleocapsid proteins were isolated and analysed by matrix-assisted laser desorption ionisation-time of flight (MALDI-ToF) mass spectrometry. Amongst the immunoglobulins, a high affinity for human serum albumin was evident in the anti-spike preparations. Careful mass comparison revealed the preferential capture of advanced glycation end product (AGE) forms of glycated human serum albumin by the pre-fusion spike protein. The ability of bacteria and viruses to surround themselves with serum proteins is a recognised immune evasion and pathogenic process. The preference of SARS-CoV-2 for AGE forms of glycated serum albumin may in part explain the severity and pathology of acute respiratory distress and the bias towards the elderly and those with (pre)diabetic and atherosclerotic/metabolic disease.
Keywords: COVID-19; convalescent plasma; glycated albumin; nucleocapsid; semi-automated magnetic rack; spike protein.
【저자키워드】 COVID-19, convalescent plasma, Spike protein, nucleocapsid, glycated albumin, semi-automated magnetic rack, 【초록키워드】 viruses, convalescent plasma, SARS-CoV-2, pathology, mass spectrometry, immune response, antibody, SARS-COV-2 infection, severity, immunoglobulins, virus, Spike protein, nucleocapsid protein, immune evasion, nucleocapsid, metabolic disease, Patient, albumin, Bacteria, serum albumin, convalescent, preference, fusion, disease, Anti-spike, binding, Anti-nucleocapsid antibodies, plasma proteins, acute respiratory distress, capture, respiratory distress, plasma protein, end product, Diabetic, Human serum albumin, Serum protein, serum proteins, atherosclerotic, high affinity, Nucleocapsid proteins, pathogenic, molecular features, flight, pre-fusion, anti-spike and anti-nucleocapsid antibodies, Complete, form, analysed, the spike protein, explain, molecular feature, 【제목키워드】 SARS-CoV-2, spike, clinical, metabolic, individual, role, Potential, Bia,