Abstract
In addition to molecular testing, there is evolving interest for anti-SARS-CoV-2 antibodies serologic assays. Majority of them focus on IgM/IgG despite IgA important role in mucosal immunity. A simultaneous anti-SARS-CoV-2 IgA/IgG/IgM immunoassay, performed on an automated instrument by ELISA kit coated with native inactivated SARS-CoV-2, was detected on two control groups (negative swab healthcare workers; pre-pandemic healthy or with other viral infections individuals) and on two COVID-19 patient groups (early and late infection). Specificities were 100% in all groups, indicating no cross-reactivity with other infectious or pre-pandemic sera. Sensitivities were 94% in early infection group and 97% in total positive patient group, reaching 100% in late infection group. To our knowledge, this is the first technique based on native SARS-CoV-2. It is able to identify more positive samples than kits using recombinant antigens, therefore virus native epitopes as well as simultaneous anti-SARS-CoV-2 IgA/IgM/IgG detection could help to contain COVID-19 spreading.
Keywords: COVID-19; Native antigens; Serological assay.
【저자키워드】 COVID-19, Serological assay, Native antigens, 【초록키워드】 SARS-CoV-2, viral infection, Immunity, knowledge, Infection, virus, anti-SARS-CoV-2, viral infections, cross-reactivity, immunoassay, anti-SARS-CoV-2 antibodies, Epitopes, Viral, Serological assay, anti-SARS-CoV-2 antibody, IgA, IgM/IgG, recombinant antigens, sera, healthcare, Patient, antigens, automated, Swab, molecular, group, epitope, serologic assays, inactivated, mucosal, early infection, COVID-19 patient, focus, automated instrument, control group, help, sensitivities, positive, ELISA kit, Specificities, positive sample, identify, performed, healthy, addition, individuals, groups, coated with, Native, 【제목키워드】 virus, anti-SARS-CoV-2, IgA, automated, inactivated, IgM and IgG,