Abstract
The E484K mutation at the SARS-CoV-2 Spike protein emerged independently in different variants around the world and has been widely associated with immune escape from neutralizing antibodies generated during previous infection or vaccination. In this work, the B.1 + L249S+E484K lineage was isolated along with A.1, B.1.420, and B.1.111 SARS-CoV-2 lineages without the E484K mutation and the neutralizing titer of convalescent sera was compared using microneutralization assays. While no significant differences in the neutralizing antibody titers were found between A.1 and B.lineages without the E484K mutation, the neutralizing titers against B.1 + L249S+E484K were 1.5, 1.9, 2.1, and 1.3-fold lower than against A.1, B.1.420, B.1.111-I, and B.1.111-II, respectively. However, molecular epidemiological data indicate that there is no increase in the transmissibility rate associated with this new lineage. This study supports the capability of new variants with the E484K mutation to be resistant to neutralization by humoral immunity, and therefore the need to intensify surveillance programs to determine if these lineages represent a risk for public health.
Keywords: COVID-19; E484k mutation; Neutralizing antibodies; SARS-CoV-2 lineages; Spike protein; Variant of Interest.
【저자키워드】 COVID-19, Neutralizing antibodies, Spike protein, variant of interest, E484K mutation, SARS-CoV-2 lineages, 【초록키워드】 neutralizing antibody, public health, SARS-CoV-2, vaccination, Neutralizing antibodies, spike, neutralization, variant, Infection, risk, Spike protein, Protein, Humoral immunity, Transmissibility, Surveillance, Immune escape, SARS-CoV-2 spike protein, Lineage, epidemiological data, molecular, E484K mutation, convalescent sera, Antibody titers, Neutralizing antibody titer, microneutralization, Neutralizing titer, B.1, Support, no significant differences, no significant difference, significant differences, neutralizing antibody titers, no increase, neutralizing titers, while, SARS-CoV-2 lineage, interest, assays, determine, the SARS-CoV-2, 【제목키워드】 neutralizing antibody, SARS-CoV-2, Lineage, convalescent,