Abstract
The development of effective vaccines against SARS-CoV-2 remains a global health priority. Despite extensive use, the effects of Sputnik V on B cell immunity need to be explored in detail. We performed comprehensive profiling of humoral and B cell responses in a cohort of vaccinated subjects (n = 22), and demonstrate that Sputnik vaccination results in robust B cell immunity. We show that B memory cell (MBC) and antibody responses to Sputnik V were heavily dependent on whether the vaccinee had a history of SARS-CoV-2 infection or not. 85 days after the first dose of the vaccine, ex vivo stimulated MBCs from the vast majority of Sputnik V vaccinees produced antibodies that robustly neutralized the Wuhan Spike-pseudotyped lentivirus. MBC-derived antibodies from all previously infected and some of the naïve vaccine recipients could also cross-neutralize Beta (B.1.351) variant of SARS-CoV-2. Virus-neutralizing activity of MBC-derived antibodies correlated well with that of the serum antibodies, suggesting the interplay between the MBC and long-lived plasma cell responses. Thus, our in-depth analysis of MBC responses in Sputnik V vaccinees complements traditional serological approaches and may provide important outlook into future B cell responses upon re-encounter with the emerging variants of SARS-CoV-2.
Keywords: COVID-19; SARS-CoV-2; Sputnik V vaccine; memory B cells; vaccination.
【저자키워드】 COVID-19, SARS-CoV-2, vaccination, memory B cells, Sputnik V vaccine, 【초록키워드】 Vaccine, vaccination, Immunity, antibody, B.1.351, SARS-COV-2 infection, Antibody Response, memory B cells, complement, B cell, Cohort, Antibody responses, Health, lentivirus, response, Wuhan, plasma, Beta, serological, Plasma cell, MBC, cross-neutralize, humoral, memory cell, B cell response, Ex vivo, subject, first dose, in-depth analysis, Effective vaccines, naïve, serum antibodies, recipient, MBCs, variants of SARS-CoV-2, Effect, responses, approach, Cell, memory B, neutralized, vaccinee, robust, variant of SARS-CoV-2, stimulated, produced, performed, majority, dependent on, correlated, effective vaccine, the vaccine, 【제목키워드】 memory B cells, induced, Produce,