Abstract
Considerable concerns relating to the duration of protective immunity against severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) exist, with evidence of antibody titers declining rapidly after infection and reports of reinfection. Here, we monitor the antibody responses against SARS-CoV-2 receptor-binding domain (RBD) for up to 6 months after infection. While antibody titers are maintained, ∼13% of the cohort’s neutralizing responses return to background. However, encouragingly, in a selected subset of 13 participants, 12 have detectable RBD-specific memory B cells and these generally are increasing out to 6 months. Furthermore, we are able to generate monoclonal antibodies with SARS-CoV-2 neutralizing capacity from these memory B cells. Overall, our study suggests that the loss of neutralizing antibodies in plasma may be countered by the maintenance of neutralizing capacity in the memory B cell repertoire.
Keywords: COSIN; COVID; RBD; RBD tetramer; SARS-CoV-2; SARS-CoV-2 pseudovirus; functional MBCs; longitudinal tracking; memory B cells; neutralizing antibodies.
【저자키워드】 SARS-CoV-2, Neutralizing antibodies, memory B cells, COVID, RBD, SARS-CoV-2 pseudovirus, COSIN, RBD tetramer, functional MBCs, longitudinal tracking, 【초록키워드】 neutralizing antibody, Neutralizing antibodies, monoclonal antibody, Infection, memory B cells, monoclonal antibodies, Severe acute respiratory syndrome, Reinfection, Cohort, Antibody responses, protective immunity, Antibody titer, plasma, neutralizing capacity, SARS-CoV-2 pseudovirus, respiratory, severe acute respiratory syndrome-coronavirus-2, Antibody titers, memory B cell, Evidence, maintenance, evidence of, Participants, domain, while, MONITOR, MBCs, Cell, memory B, selected, detectable, generate, functional, subset, the antibody response, declining, neutralizing response, 【제목키워드】 neutralizing antibody, SARS-COV-2 infection, memory B cells, persistence,