Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for an unprecedented global pandemic of COVID-19. Animal models are urgently needed to study the pathogenesis of COVID-19 and to screen vaccines and treatments. We show that African green monkeys (AGMs) support robust SARS-CoV-2 replication and develop pronounced respiratory disease, which may more accurately reflect human COVID-19 cases than other nonhuman primate species. SARS-CoV-2 was detected in mucosal samples, including rectal swabs, as late as 15 days after exposure. Marked inflammation and coagulopathy in blood and tissues were prominent features. Transcriptome analysis demonstrated stimulation of interferon and interleukin-6 pathways in bronchoalveolar lavage samples and repression of natural killer cell- and T cell-associated transcripts in peripheral blood. Despite a slight waning in antibody titers after primary challenge, enhanced antibody and cellular responses contributed to rapid clearance after re-challenge with an identical strain. These data support the utility of AGM for studying COVID-19 pathogenesis and testing medical countermeasures.
【초록키워드】 COVID-19, severe acute respiratory syndrome coronavirus 2, Transcriptome, SARS-CoV-2, Inflammation, Vaccine, coronavirus, Pathogenesis, antibody, interleukin-6, interferon, animal models, severe acute respiratory syndrome Coronavirus, Coagulopathy, Peripheral blood, global pandemic, T cell, interleukin, COVID-19 pathogenesis, Features, animal, Respiratory disease, Antibody titer, pathway, Rectal swabs, repression, respiratory, utility, African green monkey, transcriptome analysis, SARS-CoV-2 replication, Blood, Antibody titers, Stimulation, cellular response, natural killer cell, mucosal, Cellular responses, green monkeys, natural killer cell-, Support, natural killer, acute respiratory syndrome, Medical countermeasures, acute respiratory syndrome coronavirus, tissue, tissues, bronchoalveolar lavage samples, These data, COVID-19 case, African green monkeys, pathogenesis of COVID-19, green, clearance, transcript, Cell, AGMs, robust, responsible, develop, bronchoalveolar lavage sample, demonstrated, contributed, AGM, 【제목키워드】 COVID-19, Re-infection, African green monkey,