Abstract
The COVID-19 pandemic, caused by severe acute respiratory coronavirus 2 (SARS-CoV-2), has become a public health emergency and widely spread around the world. Rapid, accurate and early diagnosis of COVID-19 infection plays a crucial role in breaking this pandemic. However, the detection accuracy is limited for current single-gene diagnosis of SARS-CoV-2. Herein, we develop an autonomous lab-on-paper platform for multiplex gene diagnosis of SARS-CoV-2 by combining reverse transcription recombinase polymerase amplification (RT-RPA) and CRISPR-Cas12a detection. The autonomous lab-on-paper is capable of simultaneously detecting nucleoprotein (N) gene and spike (S) gene of SARS-CoV-2 virus as well as human housekeeping RNAse P gene (an internal control) in a single clinical sample. With the developed platform, 102 copies viral RNA per test can be detected within one hour. Also, the lab-on-paper platform has been used to detect 21 swab clinical samples and obtains a comparable performance to the conventional RT-PCR method. Thus, the developed lab-on-paper platform holds great potential for rapid, sensitive, reliable, multiple molecular diagnostics of COVID-19 and other infectious diseases in resource-limited settings.
【초록키워드】 COVID-19, SARS-CoV-2, coronavirus, pandemic, Infectious diseases, COVID-19 pandemic, SARS-CoV-2 virus, Infectious disease, RT-PCR, diagnostics, Spread, early diagnosis, CRISPR, amplification, Viral, Accuracy, COVID-19 infection, Internal control, Rapid, reverse transcription, Viral RNA, Swab, public health emergency, molecular, RT-RPA, respiratory, multiplex, platform, other infectious diseases, paper, severe acute respiratory coronavirus, polymerase, Respiratory Coronavirus, clinical sample, detect, develop, caused, comparable, autonomous, diagnosis of SARS-CoV-2, 【제목키워드】 SARS-CoV-2, diagnostics,