Abstract
The gold standard protocol for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection detection remains reverse transcription quantitative polymerase chain reaction (qRT-PCR), which detects viral RNA more sensitively than any other approach. Here, we present Homebrew, a low-cost protocol to extract RNA using widely available reagents. Homebrew is as sensitive as commercially available RNA extraction kits. Homebrew allows for sample pooling and can be adapted for automation in high-throughput settings. For complete details on the use and execution of this protocol, please refer to Page et al. (2022).
Keywords: Immunology; Microbiology; Molecular Biology.
All Keywords
【저자키워드】 immunology, Microbiology, Molecular Biology., 【초록키워드】 immunology, SARS-CoV-2, coronavirus, Microbiology, Molecular biology, protocol, qRT-PCR, Infection, severe acute respiratory syndrome Coronavirus, Automation, RNA, polymerase chain reaction, Viral, Biology, gold, reverse transcription, Viral RNA, respiratory, Quantitative, Chain Reaction, reverse transcription quantitative polymerase chain reaction, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, gold standard, reagents, RNA extraction kits, Complete, approach, polymerase chain, detect, 【제목키워드】 SARS-CoV-2, diagnostics,
【저자키워드】 immunology, Microbiology, Molecular Biology., 【초록키워드】 immunology, SARS-CoV-2, coronavirus, Microbiology, Molecular biology, protocol, qRT-PCR, Infection, severe acute respiratory syndrome Coronavirus, Automation, RNA, polymerase chain reaction, Viral, Biology, gold, reverse transcription, Viral RNA, respiratory, Quantitative, Chain Reaction, reverse transcription quantitative polymerase chain reaction, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, gold standard, reagents, RNA extraction kits, Complete, approach, polymerase chain, detect, 【제목키워드】 SARS-CoV-2, diagnostics,
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중증급성호흡기증후군 코로나바이러스 2(SARS-CoV-2) 감염 감지에 대한 표준 프로토콜은 역전사 정량적 중합효소 연쇄 반응(qRT-PCR)으로 남아 있으며, 이는 바이러스 RNA를 다른 접근 방식보다 더 민감하게 감지합니다. 여기에서는 널리 사용되는 시약을 사용하여 RNA를 추출하는 저렴한 프로토콜인 Homebrew를 소개합니다. Homebrew는 시판되는 RNA 추출 키트만큼 민감합니다. Homebrew는 샘플 풀링을 허용하고 높은 처리량 설정에서 자동화에 맞게 조정할 수 있습니다. 이 프로토콜의 사용 및 실행에 대한 자세한 내용은 Page et al. (2022).
{{ 키워드: }} 면역학; 미생물학; 분자 생물학.