Abstract
Rapid, sensitive and accurate diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is of great need for effective quarantining and treatment. Real-time reverse-transcription polymerase chain reaction requiring thermocyling has been commonly used for diagnosis of SARS-CoV-2 though it may take two to 4 h before lengthy sample pretreatment process and require bulky apparatus and well-trained personnel. Since multiple reverse transcription loop-mediated isothermal amplification (multiple RT-LAMP) process without thermocycling is sensitive, specific and fast, an electromagnetically-driven microfluidic chip (EMC) was developed herein to lyse SARS-CoV-2 viruses, extract their RNAs, and perform qualitative analysis of three marker genes by on-chip multiple RT-LAMP in an automatic format within 82 min at a limit of detection of only ∼5000 copies per reaction (i.e. 200 virus/ μL). This compact EMC may be especially promising for SARS-CoV-2 diagnostics in resource-limited countries.
Keywords: COVID-19; Microfluidics; Micromixers; Micropumps; Multiple RT-LAMP; Point-of-care.
【저자키워드】 COVID-19, Microfluidics, point-of-care., Micromixers, Micropumps, Multiple RT-LAMP, 【초록키워드】 Treatment, SARS-CoV-2, coronavirus, Diagnosis, severe acute respiratory syndrome Coronavirus, RNAs, diagnostics, Microfluidics, polymerase chain reaction, point-of-care, RT-LAMP, Loop-mediated isothermal amplification, limit of detection, Rapid, reverse transcription, isothermal amplification, respiratory, Analysis, Chain Reaction, qualitative analysis, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, reaction, SARS-CoV-2 viruses, Multiple, ChIP, effective, marker genes, marker gene, polymerase chain, diagnosis of SARS-CoV-2, 【제목키워드】 reverse transcription, platform, acute respiratory syndrome,