Abstract
Coronavirus disease 2019 is a public health challenge requiring rapid testing for the detection of infections and transmission. Nucleic acid amplification tests targeting SARS coronavirus 2 (CoV2) are used to detect CoV2 in clinical samples. Real-time reverse transcription quantitative PCR is the standard nucleic acid amplification test for CoV2, although reverse transcription loop-mediated isothermal amplification is used in diagnostics. The authors demonstrate a sequence-specific reverse transcription loop-mediated isothermal amplification-based nucleic acid amplification assay that is finished within 30 min using minimally processed clinical nasal swab samples and describe a fluorescence-quenched reverse transcription loop-mediated isothermal amplification assay using labeled primers and a quencher oligonucleotide. This assay can achieve rapid (30 min) and sensitive (1000 plaque-forming units/ml) fluorescence detection of CoV2 (WA1/2020), B.1.1.7 (Alpha) and variants of concern Delta (B.1.617.2) and Omicron (B.1.1.529) in nasal samples.
Keywords: FQ-LAMP; POC; RT-LAMP; SARS coronavirus 2; diagnostics; fluorescence; variant.
【저자키워드】 POC, diagnostics, RT-LAMP, Fluorescence, SARS coronavirus 2, Variant., FQ-LAMP, 【초록키워드】 coronavirus disease, public health, Coronavirus disease 2019, coronavirus, variant, Infection, variants of concern, Delta, B.1.617.2, Transmission, omicron, amplification, clinical samples, fluorescence detection, nucleic acid, RT-LAMP, Loop-mediated isothermal amplification, B.1.1.7, Fluorescence, Alpha, reverse transcription, isothermal amplification, B.1.1.529, nasal swab, SARS Coronavirus, nucleic acid amplification, nucleic acid amplification tests, quantitative PCR, isothermal, (alpha, sequence, WA1/2020, primer, nasal samples, detect, processed, 【제목키워드】 amplification, nasal swab,