Abstract
In the pandemic, rapid and accurate detection of SARS-CoV-2 is crucial in controlling the outbreak. Recent studies have shown a high detection rate using saliva/oral fluids as specimens for laboratory detection of the virus. We intended to evaluate the test performance of the Xpert Xpress SARS-CoV-2 cartridge assay in comparison to a conventional qRT-PCR testing, using saliva as biological specimen. Forty saliva samples from symptomatic participants were collected. Conventional qRT-PCR was performed for amplification of E and RdRp genes and the Xpert Xpress SARS-CoV-2 assay amplified E and N2 genes. In the conventional assay, the median cycle threshold value of the E gene was 34.9, and of the RdRp gene was 38.3. In the Xpert Xpress assay, the median cycle threshold value of the E gene was 29.7, and of the N2 gene was 31.6. These results can allow a broaden use of molecular tests for management of COVID-19 pandemic, especially in resources-limited settings.
Keywords: Diagnostics; GeneXpert; SARS-CoV-2; Saliva.
【저자키워드】 SARS-CoV-2, Saliva, diagnostics, GeneXpert, 【초록키워드】 Saliva, pandemic, COVID-19 pandemic, qRT-PCR, virus, Laboratory, amplification, outbreak, management, RdRP, detection rate, Molecular tests, RdRp gene, Cycle threshold value, E gene, specimen, symptomatic participants, molecular test, cartridge, Genes, recent, RdRp genes, shown, amplified, collected, evaluate, was performed, Conventional, symptomatic participant, the median, 【제목키워드】 validation, SARS-CoV-2 PCR, specimen,