Abstract
Ultrasensitive, versatile sensors for molecular biomarkers are a critical component of disease diagnostics and personalized medicine as the COVID-19 pandemic has revealed in dramatic fashion. Integrated electrical nanopore sensors can fill this need via label-free, direct detection of individual biomolecules, but a fully functional device for clinical sample analysis has yet to be developed. Here, we report amplification-free detection of SARS-CoV-2 RNAs with single molecule sensitivity from clinical nasopharyngeal swab samples on an electro-optofluidic chip. The device relies on optically assisted delivery of target carrying microbeads to the nanopore for single RNA detection after release. A sensing rate enhancement of over 2,000x with favorable scaling towards lower concentrations is demonstrated. The combination of target specificity, chip-scale integration and rapid detection ensures the practicality of this approach for COVID-19 diagnosis over the entire clinically relevant concentration range from 10 4 -10 9 copies/mL.
Keywords: Biosensors; Nasopharyngeal swab samples; SARS-CoV-2 RNAs; Single-molecule detection; Solid-state nanopores.
【저자키워드】 biosensors, Nasopharyngeal swab samples, SARS-CoV-2 RNAs, Single-molecule detection, Solid-state nanopores., 【초록키워드】 SARS-CoV-2, Biomarker, COVID-19 pandemic, Nanopore, RNAs, diagnostics, RNA, Nasopharyngeal swab, amplification, sensitivity, nasopharyngeal, Personalized medicine, COVID-19 diagnosis, target, SARS-CoV-2 RNA, Swab, molecular, disease, Critical, Nasopharyngeal swab samples, Device, Combination, Concentration, Analysis, assisted delivery, nasopharyngeal swab sample, target specificity, approach, practicality, clinically, functional, demonstrated, electrical, 【제목키워드】 SARS-CoV-2 RNA,