Abstract
The outbreak caused by the novel coronavirus SARS-CoV-2 has been declared a global health emergency. G-quadruplex structures in genomes have long been considered essential for regulating a number of biological processes in a plethora of organisms. We have analyzed and identified 25 four contiguous GG runs (G2NxG2NyG2NzG2) in the SARS-CoV-2 RNA genome, suggesting putative G-quadruplex-forming sequences (PQSs). Detailed analysis of SARS-CoV-2 PQSs revealed their locations in the open reading frames of ORF1 ab, spike (S), ORF3a, membrane (M) and nucleocapsid (N) genes. Identical PQSs were also found in the other members of the Coronaviridae family. The top-ranked PQSs at positions 13385 and 24268 were confirmed to form RNA G-quadruplex structures in vitro by multiple spectroscopic assays. Furthermore, their direct interactions with viral helicase (nsp13) were determined by microscale thermophoresis. Molecular docking model suggests that nsp13 distorts the G-quadruplex structure by allowing the guanine bases to be flipped away from the guanine quartet planes. Targeting viral helicase and G-quadruplex structure represents an attractive approach for potentially inhibiting the SARS-CoV-2 virus.
Keywords: Coronaviridae; G-quadruplex; PQSs; SARS-CoV-2; helicase inhibitor; viral helicase nsp13.
【저자키워드】 SARS-CoV-2, G-quadruplex, Coronaviridae, PQSs, helicase inhibitor, viral helicase nsp13., 【초록키워드】 Structure, Genome, G-quadruplex, SARS-CoV-2 virus, docking, in vitro, Novel coronavirus, RNA, Helicase, nsp13, Viral, ORF3a, outbreak, nucleocapsid, membrane, genomes, Coronaviridae, Interaction, Open reading frame, Analysis, open reading frames, determined by, health emergency, Organisms, biological processes, novel coronavirus SARS-CoV-2, sequence, base, Coronaviridae family, RNA genome, plethora, ORF1, guanine, targeting, Genes, approach, analyzed, caused, assays, inhibiting, the Coronaviridae, biological processe, the SARS-CoV-2, the SARS-CoV-2 virus, 【제목키워드】 sequence,