Abstract
Background: High cost of commercial RNA extraction kits limits the testing efficiency of SARS-CoV-2. Here, we developed a simple nucleic acid extraction method for the detection of SARS-CoV-2 directly from nasopharyngeal swab samples.
Methods: A pH sensitive dye was used as the end point detection method. The obvious colour changes between positive and negative reactions eliminates the need of other equipment.
Results: Clinical testing using 260 samples showed 92.7% sensitivity (95% CI 87.3-96.3%) and 93.6% specificity (95% CI 87.3-97.4%) of RT-LAMP.
Conclusions: The simple RNA extraction method minimizes the need for any extensive laboratory set-up. We suggest combining this simple nucleic acid extraction method and RT-LAMP technology as the point-of care diagnostic tool.
Keywords: Diagnosis; Phenol red; RT-LAMP; SARS-CoV-2; pH sensitive indicator.
【저자키워드】 SARS-CoV-2, Diagnosis, RT-LAMP, Phenol red, pH sensitive indicator., 【초록키워드】 equipment, diagnostic, Laboratory, RNA extraction, Nasopharyngeal swab, nucleic acid, RT-LAMP, sensitivity, specificity, clinical, change, Care, Nasopharyngeal swab samples, Phenol red, Efficiency, phenol, 95% CI, reaction, nucleic acid extraction, end point, clinical testing, positive, limit, sensitive indicator, was used, RNA extraction kit, 【제목키워드】 clinical, isothermal amplification, SARS-CoV-2 swab sample,