We detected the virus genome in ocular samples from a 65-year-old woman with clinically diagnosed acute retinal necrosis using DNA amplification. She exhibited occlusive retinal vasculitis, confluent necrotizing retinitis, mainly peripheral, and iridocyclitis. For DNA amplification, we used recently published primers specific for varicella-zoster virus (VZV) and herpes simplex virus. Using VZV primers, we detected the VZV genome in the aqueous humor, but not in the vitreous, by amplifying a DNA fragment 642 base pairs in length. HSV DNA was not detected. After detecting the VZV genome, PstI restriction endonuclease was used because an epidemiological study found that about 25% of the VZV strains in Japan carry a mutation lacking a PstI recognition site. The VZV genome from the patient had a PstI cleavage pattern, while the positive control had a VZV genome that carried a PstI-site-less mutation. We considered our patient with acute retinal necrosis to be infected with VZV having a PstI site.
Detection of varicella-zoster virus genome having a PstI site in the ocular sample from a patient with acute retinal necrosis
급성 망막 괴사 환자의 안구 샘플에서 PstI 부위를 가진 수두대상포진 바이러스 유전체의 검출
[Category] 대상포진, 두창, 수두,
Pubmed URL [DOI] 10.1159/000267740
[Article Type] Case Reports
[Source] pubmed
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