Abstract
Objectives: SARS-CoV-2 exhibits tropism for the gastrointestinal tract; however, lesions in enterocytes and their correlation with disease severity and patient prognosis are still unknown.
Methods: SARS-CoV-2 patients were enrolled in 5 medical centres in São Paulo, Brazil and their clinical characteristics and laboratory findings recorded. At admission, day 7 and day 14 of hospitalisation, plasma and urine samples were collected, and cytokine levels and intestinal fatty acid-binding protein (I-FABP) concentrations measured.
Results: COVID-19 patients displayed ≈48-, 74- and 125-fold increased urinary I-FABP levels at admission (n=283; P<0.001), day 7 (n=142; P<0.01) and day 14 (n=75; P<0.01) of hospitalisation. Critically ill patients and nonsurvivors showed higher I-FABP concentrations compared with patients with less severe illness. At admission, infected patients demonstrated enhanced production of plasma interferon (IFN)-γ and interleukin (IL)-6. The receiver operating characteristic curve suggested I-FABP as a biomarker for COVID-19 disease severity at admission (P<0.0001; Youden index=6.89; area under the curve=0.699). Patients with I-FABP ≥6.89 showed higher IL-6 and C-reactive protein levels (P<0.001) at admission and had a prolonged length of hospital stay.
Conclusions: Our findings revealed damage to enterocytes in SARS-CoV-2 infection, which is associated with illness severity, poor prognosis and exacerbated inflammatory response.
Keywords: COVID-19; cytokines; inflammation; interleukin-6; intestinal epithelial cells.
【저자키워드】 COVID-19, Inflammation, Cytokines, interleukin-6, intestinal epithelial cells., 【초록키워드】 Brazil, SARS-CoV-2, Biomarker, Cytokines, Prognosis, Clinical characteristics, IL-6, SARS-COV-2 infection, disease severity, interleukin-6, interferon, C-reactive protein, cytokine, Laboratory, laboratory findings, COVID-19 disease, Protein, interleukin, Critically ill, Patient, Urine, plasma, epithelial cells, correlation, characteristic, hospitalisation, Admission, gastrointestinal tract, critically ill patients, cytokine levels, Fatty acid, COVID-19 patients, Inflammatory response, Concentration, COVID-19 patient, enterocytes, Hospital stay, Illness severity, receiver operating characteristic, Critically ill patient, lesion, infected patients, damage, nonsurvivors, lesions, poor prognosis, receiver operating characteristic curve, COVID-19 disease severity, intestinal epithelial cells, intestinal, enrolled, collected, exacerbated, less, demonstrated, suggested, infected patient, exhibit, recorded, Enterocyte, cytokine level, fatty, nonsurvivor, 【제목키워드】 Protein, clinical, intestinal, fatty,